Isolating RNA without using DNase??

Stefan Wirtz wirts000 at mzdmza.zdv.uni-mainz.de
Fri Nov 8 11:01:54 EST 1996


In article <55u4sh$d4h at news.iastate.edu>, qfdong at iastate.edu (Qunfeng
Dong) wrote:

> Hi, there:
> 
>   When I read the "current protocols in molecular biology", I am curious why
> those RNA isolation procedures don't need to use DNase in any step, how is
> DNA removed during the isolation??
> 

Some protocols for RNA extraction use NP-40 to lyse the cells. This
reagent (in the right concentration) destroys the cell membrane but leaves
the nuclear membrane intact. That allows you to isolate RNA without any
contamination of DNA.

Christoph Becker, University Mainz, Germany



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