Soubilizing protein pellet for liquid scintillation counting

huang at sun5.ibp.ac.cn huang at SUN5.IBP.AC.CN
Mon Nov 18 22:25:42 EST 1996


Hi all!

I have several hundred microgram protein pellet
bound by a tritium-labeled polar ligand. 
Ordering for a commercial available solubilizer is 
very inconvenience  and time-consumed in China.
Could anyone give me a suggestion on how to
solubilize the sample to make it counted in
xylene-based scintillation cocktail efficiently?

Thank you in advance.

Guang Hu
Master candidate in
Institute of Biophysics, Science Academy of P.R. China
Huang at sun5.ibp.ac.cn





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