Nonfat Milk Powder & Western Blot

Anonymous nobody at REPLAY.COM
Mon Nov 18 05:41:11 EST 1996


mmmm

In article <jnakamot-1811960147130001 at news.ucla.edu>, jnakamot at ucla.edu
(Jon Nakamoto) wrote:

> Whatever you decide to use, I'd run a comparison of two or three milk
> powders beforehand. I remember how variable results could be (Carnation
> looked more yellowish than the others and gave horrible results; Sanalac
> and Ralphs market's generic brand worked great). 
> 
> Jon
> 
> Jon Nakamoto, MD
> Asst Prof of Pediatrics/Endocrinology
> UCLA Children's Hospital
> jnakamot at ucla.edu
> 
> 
> 
> 
> In article <32879DB4.BD8 at indi.aci.uni-heidelberg.de>,
> stefan at indi.aci.uni-heidelberg.de wrote:
> 
> > Hi Bionet Folks.
> > 
> > I'd like to use milk powder in a western blot instead of bsa (bovine
> > serum albumine) for the blocking of the membrane.
> > 
> > As far as I know nonfat milk powder has to be used for that purpose.
> > Here in Germany I could get low fat powder that still contains 1 % fat.
> > 
> > So the question is: What is nonfat milk powder? Does it contain zero %
> > fat? Or does it just mean to contain only 1 % so that the stuff I got
> > here would be ok?
> > 
> > Does anybody know? Or at least have an idea where I could get a hint if
> > not here?
> > 
> > Best regards
> > Stefan
> > 
> > Stefan Beyreuther
> > Inorganic Chemistry Department
> > University of Heidelberg
> > Germany
> 
> -- 
> Jon Nakamoto
> jnakamot at ucla.edu



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