Prep of DNA for electroporation

John Brennand john.brennand at gbapr.zeneca.com
Mon Nov 18 07:43:50 EST 1996


i agree with dima - the salt is the problem

we process the ligations thus:-

add 2 ul strataclean resin vortex, spin.

transfer the solution to a whatman microdialysis membrane floating 
on a petri dish of water. leave for an hour or so - transfer to 
fresh tube for electroporation.

this works every time - no arcing and better frequencies than with 
precipitated dna (we did a head to head experiment to test this)

john



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