Prep of DNA for electroporation
john.brennand at gbapr.zeneca.com
Mon Nov 18 07:43:50 EST 1996
i agree with dima - the salt is the problem
we process the ligations thus:-
add 2 ul strataclean resin vortex, spin.
transfer the solution to a whatman microdialysis membrane floating
on a petri dish of water. leave for an hour or so - transfer to
fresh tube for electroporation.
this works every time - no arcing and better frequencies than with
precipitated dna (we did a head to head experiment to test this)
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