Autoclaving Reagents

David Winterbourne sghk100 at
Mon Nov 18 06:20:30 EST 1996

In article <328FB6DA.6DEA at>, Rick Bright says...

> during the autoclave procedure, the bottle
>containing the pH 7.6 split around the bottom.  The entire bottom was
>cut all around the bottle, so the top just lifted off and the solution
>went happily down the drain.

This is caused by rapid heating of the glass in the autoclave, followed by 
cooling due to convection in the solution.

You can prevent bottles from cracking by preheating the solution in the bottle 
to 50-60C in a microwave.

Dr. David Winterbourne
Department of Surgery
St George's Hospital Medical School, London SW17 0RE, England
Tel: 0181-725-5581   Fax: 0181-725-3594

More information about the Methods mailing list