Prep of DNA for electroporation

Dr. Duncan Clark duncan at genesys.demon.co.uk
Tue Nov 19 09:15:53 EST 1996


As reported in NAR and other places, salt and T4 DNA ligase kills the
electroporation efficiency. 

Take a 10ul ligation, add 20ul water, heat at 70C for 20 mins, briefly
microfuge then zap 1 or 2ul. No problem with arcing or low efficiency.

Duncan


--------------------------------------------------------------------------------
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Dr. Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288
http://www.prestel.co.uk/dnamp
http://www.genesys.demon.co.uk



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