Electroporation cuvettes

Kimberley Snowden kcsnowden at ucdavis.edu
Wed Nov 20 21:52:00 EST 1996

rgrant at worf.molbiol.ox.ac.uk (Richard P Grant) wrote:

>Possibly the autoclave.  Don't laugh - plasmid DNA survives autoclaving
>remarkably well.  The autoclave must be the most efficient method of
>spreading ccDNA around the place known to man.  When we make competent
>cells, the glassware we use does not go anywhere near the autoclave or the
>washing up machine.

I am curious then how you sterilise your glassware for this.  Ethanol,
bleach or UV or a combination?  Or something I haven't thought of?

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