kcsnowden at ucdavis.edu
Wed Nov 20 21:52:00 EST 1996
rgrant at worf.molbiol.ox.ac.uk (Richard P Grant) wrote:
>Possibly the autoclave. Don't laugh - plasmid DNA survives autoclaving
>remarkably well. The autoclave must be the most efficient method of
>spreading ccDNA around the place known to man. When we make competent
>cells, the glassware we use does not go anywhere near the autoclave or the
>washing up machine.
I am curious then how you sterilise your glassware for this. Ethanol,
bleach or UV or a combination? Or something I haven't thought of?
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