long pcr

susan stepp stepp at utsw.swmed.edu
Wed Nov 20 15:42:45 EST 1996


i've been using the elongase kit from BRL for a couple of years with
great success to PCR out to 16kb.  EXCEPT for the last two months
NOTHING has worked - i get no PCR product at all.  all the usual
suspects have been investigated, with no success.  does anyone have
ideas as far as something subtle that might be going wrong?  or does
anyone have any experience with this system that might help me?

thanks in advance,
sue



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