2.4 kb PCR

Rachelle Leger ax041 at FreeNet.Carleton.CA
Wed Nov 20 11:49:09 EST 1996


I am trying to do a 2.4 KB PCR on genomic DNA. I tried once with an extension
time of two minutes. I got nothing.  I know that the primers work because I
used them on an RT PCR reaction.  I use plain TAQ DNA polymerase.
Any advice?

Regards
Rachelle



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