Help: Desalting Oligos
The Great American Gene Company
geneco at ix.netcom.com
Wed Nov 20 12:14:56 EST 1996
There is only one method you can use that will ensure that you do not
lose much of your DNA. It is the subject of a short paper by Freie and
Larsen, in BioTechniques (1991) Vol 10, Page 420. The paper describes
recovery of oligos in milligram quantities. You should scale back and
rather than use a 900 mg Sep Pak, use a 250 mg or so. We have used
this for years to desalt synthetic oligos and it works excellently.
You can expect to lose no more than about 5 - 10%.
Mike MacDonell, Ph.D.
Ransom Hill Bioscience/Great American Gene Company
More information about the Methods