Doxycycline stable in H2O?

iddavis at vms.cis.pitt.edu iddavis at vms.cis.pitt.edu
Thu Nov 21 11:15:40 EST 1996


In article <BarryMook-2011961743530001 at johnsond3.med.yale.edu>, 
BarryMook at aol.com (Barry Mook) writes:
>    I'm working on a Doxycycline inducible protein expression system.  I
> keep my Doxy at 1 ug/ml in water....but how stable is it??  Does anyone
> know how long you can keep Dox in a aqueous stock solution before it loses
> activity?

It's more stable than common or garden variety tetracycline but still not very.
You should keep aliquots frozen and thaw for use.  Change your culture medium
every few days.  Change water bottles daily if using in vivo.

Ian Davis				iddavis at vms.cis.pitt.edu








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