Q: pGL3basic: Ligation TIME and TEMPERATURE??

jstrassw at OPAL.TUFTS.EDU jstrassw at OPAL.TUFTS.EDU
Thu Nov 21 10:51:22 EST 1996


Hello!
Man isch ta nah?  (so much form my hebrew)
I use PGL2 (someone said its better than pGL3 serties but I dont remember=
=20
why.  There should be no difference in cloning with this than with other=20
vectors.  I usually ligate for 20 minutes benchtop and transform.  Works=20
Great.

John


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John Strasswimmer, MD,PhD Candidate    | Phone (617) 636 8396
Tufts - New England Medical Center     | Fax   (617) 636 6190
Box 166                                | Email:  jstrassw at opal.tufts.edu
Boston, MA 02111 USA                   |                             =20
     HTTP://WWW.healthsci.tufts.edu/microbiology/strass/JSpage.HTML
     *** Co-Author of "HyperBug" Microbiology Teaching Software ***
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Remember, Your employer is eavesdropping... =20
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On Thu, 21 Nov 1996, Philippe Lefebvre wrote:

> My TIME/TEMPERATURE settings for:
> > a) religation of the pGL3basic vector
> > b) ligation of an insert into the pGL3basic vector
> > would be for both one hour at 25 =B0C
>=20
> Dr Phil Good
>=20
>=20



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