In situ PCR of bacterial colonies?

[eric anderson]

In article <goldberg-2211961413090001 at 140.176.74.203>, goldberg at bms.com
(Steven Goldberg) wrote:

> I was just wondering if anyone has developed or heard about an in situ PCR
> protocol for E. coli colonies carrying cloned genes.  If you have any
> information on this topic I would like to hear from you.

steve,

if you mean performing PCR on a colony to see if your insert is present, i
do it all the time.  e-mail me for details (which are pretty minimal) if
you like.

eric

-- 
Eric C. Anderson
Memorial Sloan-Kettering Cancer Center
Sloan-Kettering Institute
1275 York Ave. Box 470
New York, NY  10028
(212) 639-2977
e-anderson at ski.mskcc.org