mRNA prep with Promega Kit

Noel Fong ez005881 at dale.ucdavis.edu
Fri Nov 22 01:02:56 EST 1996


	I've been trying to prepare mRNA from total RNA using Promega's
PolyATract mRNA Isolation Kit.  From 1 mg of Total RNA, I get nothing
(expected = 10-20 ug).  My labmate has had low yields with this kit in the
past.  
	I'm working with Promega to troubleshoot the problem, but I want
to know what experiences you've had with this kit.  How does it compare to
other kits?

	Thx, Noel



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