RNA purification question !!!!!!!

Volker Blaschke vblasch at gwdg.de
Fri Nov 22 07:06:34 EST 1996

For liver RNA preparation, we lyse in GTC and then ultracentrifuge with 
a CsCL cushion. For removal of glycogen, our lab manual says to wash the 
NaAc/EtOH-precipitated RNA pellet with 3 M NaAc twice (with 
centrifugation)followed by two washes with 80 % EtOH. 

Good luck,


Dr. Volker Blaschke
Depts. of Biochemistry, Dermatology
Georg-August-University, Goettingen, Germany
Email: vblasch at gwdg.de
Tel: xx49 551 39 5959 / 5978

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