Electropoation of lympocytes and liver cells

Philip Ta-Chih Cheng tcheng at tiger.lsu.edu
Sat Nov 23 15:59:37 EST 1996


Hi, everyone,

I am new to electroporate the lympocytes (B,T,& marcrophages)and the
liver cells of fish.  I encounter two problems:

	1. How can I estamate the electroporation efficiency ?
	2. What is the critical factors of the electroporation to the 	  
lymphocytes and liver cells of fish ?

Any suggestion and information about electroporation of these cells will
be appreciated.

Thank you.


Philip Cheng

Department of Veterinary Microbiology and Parasitology
Louisiana State University




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