AmpliTaq Gold

Ted Michelini tedm at darkwing.uoregon.edu
Mon Nov 25 01:04:16 EST 1996


In article <56vtob$ehv at news.myriad.net>, immgen at immgen.com (ImmGen, Inc.) wrote:

> Anyone have experience using AmpliTaq Gold from Perkin Elmer?  Specifically, 
> does it seem to cut down on background/nonspecific bands when used in 
> multiplex PCR?
> 
> Thanks for your help.
> 
> Jennifer

Apparently so Jennifer, but probably no cleaner than a good hotstart. Its
easier and no contamination worry. They use a heat labile crosslink to
inactivate the Taq pol until after the initial denature, according to
trustworthy rumors. There are cheaper ways to do this and hasn't PE/Cetus
made enough money off PCR already? I'd test with a good hotstart and see
if its worth it to go for the "Gold".

regards
Ted Michelini
Institute of Molecular Biology
University of Oregon



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