DNA Quantification

Otter otterpop at pilot.msu.edu
Mon Nov 25 02:34:27 EST 1996


In article <elauzier-2311961202480001 at mac106.peps.ulaval.ca>,
elauzier at fse.ulaval.ca (Edouard Lauzier) wrote:

> In article (Dans l'article) <3297162E.6048 at mnw.net>, Rick Bright
> <rbright at mnw.net> wrote (écrivait) :
> 
> > I have extracted DNA from whole blood and have checked the purity using
> > the 260/280 ratio.  My professor then asked me how much DNA I extracted,
> > in micrograms.  Stumped as I was, I thought I would ask for a method or
> > equation that would tell me this.  A rough estimate is fine, is there is
> > an easy technique or formula.  Thanks.
> 
> 
> Well,
> 
> 1 A260 unit of double stranded DNA = 50 micrograms/microliter ( single
> stranded = 33 )

**I think he's wrong; I think s.s. = 40 not 33.  You can look this up in a
text book to make sure..

> Knowing your dilution, you can get the [ ] of your sample and then you
> multiply by the volume and bang ! it's done...
> 
> Bye
> -- 
> Edouard Lauzier, B.Sc.                           elauzier at fse.ulaval.ca
> Physical Activity Science Laboratory       (418) 656-2131 #2929
> (Laval University)  G1K 7P4
> CANADA



More information about the Methods mailing list