Have you heard of this: In vivo deletions of pCRII

Steven Goldberg goldberg at bms.com
Mon Nov 25 13:02:29 EST 1996


A member of our lab recently cloned a ca.100 bp PCR fragment into the
Invitrogen vector pCRII.  The fragment was present in the white colonies
that were screened but upon sequencing he found that a 600 bp deletion had
also taken place.  The deletion extended from just downstream of one of
the EcoRI sites in this vector and encompassed most of the F1 ori.  Has
anyone ever experience this phenomenon or could speculate what happened?  

Steve



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