2 min/Kb for Pfu polymerase

KHUANG at CHEMVX.TAMU.EDU KHUANG at CHEMVX.TAMU.EDU
Mon Nov 25 12:51:26 EST 1996


Hi, Netters,
Does anybody out there can tell me why the extension time for pfu polymerase
is 2 min/kb in PCR reaction? According to Stratagene Protocol, the extension 
time at least is 2 min/kb of PCR target.
Is there any relationship between extension time and PCR yield? 
Thanks!

Huang



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