help dd-PCR

Louis Alarcon lha at
Mon Nov 25 12:20:04 EST 1996

In article <57bu0v$64i8 at>, xie at
(Youhua Xie) wrote:

> Hi 
> I am going to use DD-PCR to search for a new gene. I want to know which 
> reverse transcriptase and reaction conditions
> I should use to get longer 1 strand cDNAs. 
> Thank you
> xie

We use Superscript RT (Gibco).
Reference: Mou et al, BBRC 199(2):564, 1994.
Works like a charm.

Louis Alarcon, MD
University of Pittsburgh
Department of Surgery

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