help dd-PCR

[Louis Alarcon]

In article <57bu0v$64i8 at sat.ipp-garching.mpg.de>, xie at made.biochem.mpg.de
(Youhua Xie) wrote:

> Hi 
> 
> I am going to use DD-PCR to search for a new gene. I want to know which 
> reverse transcriptase and reaction conditions
> I should use to get longer 1 strand cDNAs. 
> Thank you
> 
> xie

We use Superscript RT (Gibco).
Reference: Mou et al, BBRC 199(2):564, 1994.
Works like a charm.

Louis Alarcon, MD
University of Pittsburgh
Department of Surgery
http://www3.pitt.edu/~lalarcon/