lha at med.pitt.edu
Mon Nov 25 12:20:04 EST 1996
In article <57bu0v$64i8 at sat.ipp-garching.mpg.de>, xie at made.biochem.mpg.de
(Youhua Xie) wrote:
> I am going to use DD-PCR to search for a new gene. I want to know which
> reverse transcriptase and reaction conditions
> I should use to get longer 1 strand cDNAs.
> Thank you
We use Superscript RT (Gibco).
Reference: Mou et al, BBRC 199(2):564, 1994.
Works like a charm.
Louis Alarcon, MD
University of Pittsburgh
Department of Surgery
More information about the Methods