DNA Quantification

The Great American Gene Company geneco at ix.netcom.com
Mon Nov 25 11:33:30 EST 1996


The number cited (33 ug) is approximately correct.  The value is
sequence dependent and we use a slightly more conservative 31 ug/ODU. 
But it is not 40.

-Mike MacDonell, Ph.D.

In <otterpop-ya023180002511960234270001 at news.msu.edu>
otterpop at pilot.msu.edu (Otter) writes: 
>
>In article <elauzier-2311961202480001 at mac106.peps.ulaval.ca>,
>elauzier at fse.ulaval.ca (Edouard Lauzier) wrote:
>
>> In article (Dans l'article) <3297162E.6048 at mnw.net>, Rick Bright
>> <rbright at mnw.net> wrote (écrivait) :
>> 
>> > I have extracted DNA from whole blood and have checked the purity
using
>> > the 260/280 ratio.  My professor then asked me how much DNA I
extracted,
>> > in micrograms.  Stumped as I was, I thought I would ask for a
method or
>> > equation that would tell me this.  A rough estimate is fine, is
there is
>> > an easy technique or formula.  Thanks.
>> 
>> 
>> Well,
>> 
>> 1 A260 unit of double stranded DNA = 50 micrograms/microliter (
single
>> stranded = 33 )
>
>**I think he's wrong; I think s.s. = 40 not 33.  You can look this up
in a
>text book to make sure..
>
>> Knowing your dilution, you can get the [ ] of your sample and then
you
>> multiply by the volume and bang ! it's done...
>> 
>> Bye
>> -- 
>> Edouard Lauzier, B.Sc.                          
elauzier at fse.ulaval.ca
>> Physical Activity Science Laboratory       (418) 656-2131 #2929
>> (Laval University)  G1K 7P4
>> CANADA

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