DNA Quantification

Hiranya Roychowdhury hroychow at NMSU.EDU
Mon Nov 25 11:23:44 EST 1996


At 05:02 PM 11/23/96 GMT, Edouard Lauzier wrote:
>In article (Dans l'article) <3297162E.6048 at mnw.net>, Rick Bright
><rbright at mnw.net> wrote (=E9crivait)=A0:
>
>> I have extracted DNA from whole blood and have checked the purity using
>> the 260/280 ratio.  My professor then asked me how much DNA I extracted,
>> in micrograms.  Stumped as I was, I thought I would ask for a method or
>> equation that would tell me this.  A rough estimate is fine, is there is
>> an easy technique or formula.  Thanks.
>
>
>Well,
>
>1 A260 unit of double stranded DNA =3D 50 micrograms/microliter ( single
>stranded =3D 33 )

There you go again Ed! Or, may be the above was an honest mistake (or a=
 typo?)


>
>Knowing your dilution, you can get the [ ] of your sample and then you
>multiply by the volume and bang ! it's done...
>
>Bye
>--=20
>Edouard Lauzier, B.Sc.                           elauzier at fse.ulaval.ca
>Physical Activity Science Laboratory       (418) 656-2131 #2929
>(Laval University)  G1K 7P4
>CANADA
>
>
>
>
>Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu



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