DNA Quantification

Dr. Michael T. MacDonell sendero at ix.netcom.com
Tue Nov 26 14:19:42 EST 1996


Then you are using the wrong number!
You could use 50, but it would be wrong, too.
There are approximately 31 micrograms of ssDNA per ODU.

Best Regards!
Mike MacDonell

In <E1H1DC.7AD at cf.ac.uk> Joe Boutell <boutell at cf.ac.uk> writes: 
>
>geneco at ix.netcom.com(The Great American Gene Company ) wrote:
>>The number cited (33 ug) is approximately correct.  The value is
>>sequence dependent and we use a slightly more conservative 31 ug/ODU.

>>But it is not 40.
>>
>>-Mike MacDonell, Ph.D.
>>
>>In <otterpop-ya023180002511960234270001 at news.msu.edu>
>>otterpop at pilot.msu.edu (Otter) writes: 
>>>
>>>In article <elauzier-2311961202480001 at mac106.peps.ulaval.ca>,
>>>elauzier at fse.ulaval.ca (Edouard Lauzier) wrote:
>>>
>>>> In article (Dans l'article) <3297162E.6048 at mnw.net>, Rick Bright
>>>> <rbright at mnw.net> wrote (écrivait) :
>>>> 
>>>> > I have extracted DNA from whole blood and have checked the
purity
>>using
>>>> > the 260/280 ratio.  My professor then asked me how much DNA I
>>extracted,
>>>> > in micrograms.  Stumped as I was, I thought I would ask for a
>>method or
>>>> > equation that would tell me this.  A rough estimate is fine, is
>>there is
>>>> > an easy technique or formula.  Thanks.
>>>> 
>>>> 
>>>> Well,
>>>> 
>>>> 1 A260 unit of double stranded DNA = 50 micrograms/microliter (
>>single
>>>> stranded = 33 )
>>>
>>>**I think he's wrong; I think s.s. = 40 not 33.  You can look this
up
>>in a
>>>text book to make sure..
>>>
>>>> Knowing your dilution, you can get the [ ] of your sample and then
>>you
>>>> multiply by the volume and bang ! it's done...
>>>> 
>>>> Bye
>>>> -- 
>>>> Edouard Lauzier, B.Sc.                          
>>elauzier at fse.ulaval.ca
>>>> Physical Activity Science Laboratory       (418) 656-2131 #2929
>>>> (Laval University)  G1K 7P4
>>>> CANADA
>>
>>-- 
>>+-----------------------------------------------------+
>>| 101010  1120  222  132  110  60  52  46  42  39  33 |
>>+-----------------------------------------------------+
>>
>>
>I use 40 for quantifying RNA, 50 for dsDNA.
>Cheers,
>Joe Boutell.
>

-- 
+-----------------------------------------------------+
| 101010  1120  222  132  110  60  52  46  42  39  33 |
+-----------------------------------------------------+





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