TE Buffer for RNA

Franek, Karl - Microbiology kfrane at lsumc.edu
Tue Nov 26 12:08:27 EST 1996

In making TE buffer for RNA use is it common practice to treat freshly =
made up TE with DEPC?  I've heard that Tris interacts with DEPC =
unfavorably therby negating the RNase-inactiving effects of the latter - =
although I've never seen this documented.  If this is true, can someone =
suggest how RNase-free TE can be made?
Many thanks!

Karl Franek
Microbiology & Immunology
LSU Medical Center
Shreveport, LA 71130

kfrane at mail.sh.lsumc.edu

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