DNA Quantification

Joe Boutell boutell at cf.ac.uk
Tue Nov 26 04:36:48 EST 1996


geneco at ix.netcom.com(The Great American Gene Company ) wrote:
>The number cited (33 ug) is approximately correct.  The value is
>sequence dependent and we use a slightly more conservative 31 ug/ODU. 
>But it is not 40.
>
>-Mike MacDonell, Ph.D.
>
>In <otterpop-ya023180002511960234270001 at news.msu.edu>
>otterpop at pilot.msu.edu (Otter) writes: 
>>
>>In article <elauzier-2311961202480001 at mac106.peps.ulaval.ca>,
>>elauzier at fse.ulaval.ca (Edouard Lauzier) wrote:
>>
>>> In article (Dans l'article) <3297162E.6048 at mnw.net>, Rick Bright
>>> <rbright at mnw.net> wrote (écrivait) :
>>> 
>>> > I have extracted DNA from whole blood and have checked the purity
>using
>>> > the 260/280 ratio.  My professor then asked me how much DNA I
>extracted,
>>> > in micrograms.  Stumped as I was, I thought I would ask for a
>method or
>>> > equation that would tell me this.  A rough estimate is fine, is
>there is
>>> > an easy technique or formula.  Thanks.
>>> 
>>> 
>>> Well,
>>> 
>>> 1 A260 unit of double stranded DNA = 50 micrograms/microliter (
>single
>>> stranded = 33 )
>>
>>**I think he's wrong; I think s.s. = 40 not 33.  You can look this up
>in a
>>text book to make sure..
>>
>>> Knowing your dilution, you can get the [ ] of your sample and then
>you
>>> multiply by the volume and bang ! it's done...
>>> 
>>> Bye
>>> -- 
>>> Edouard Lauzier, B.Sc.                          
>elauzier at fse.ulaval.ca
>>> Physical Activity Science Laboratory       (418) 656-2131 #2929
>>> (Laval University)  G1K 7P4
>>> CANADA
>
>-- 
>+-----------------------------------------------------+
>| 101010  1120  222  132  110  60  52  46  42  39  33 |
>+-----------------------------------------------------+
>
>
I use 40 for quantifying RNA, 50 for dsDNA.
Cheers,
Joe Boutell.




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