Thermostable Klenow

Ashok Aiyar aiyar at ebv.oncology.wisc.edu
Wed Nov 27 11:15:39 EST 1996


On Wed, 27 Nov 1996 14:10:10 +0000, Dr. Duncan Clark <duncan at genesys.demon.co.uk> wrote:
>In article <jkropp-2611961242300001 at 128.218.128.248>, Jerry Kropp
><jkropp at itsa.ucsf.edu> writes
>>Hi Netters:
>>Anyone out there know of a source of thermostable Klenow (if that's not an
>>oxymoron!)?
>
>Klenow has both polymerase and 3'-5' proofreading exonuclease. You can
>get KlenTaq which is thermophilic but has no 3'-5' exo. Pfu and Pwo
>should be functionally equivalent to Klenow.

Or Vent or DeepVent.  I do not know how the error rates of any of these
(Vent, D.Vent, Pfu, Pwo) compare to the error rate of Klenow.

Ashok

-- 
Ashok Aiyar, Ph.D.
McArdle Laboratory for Cancer Research
University of Wisconsin-Madison





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