baffling pBluescript stuff...
drm21 at mole.bio.cam.ac.uk
Wed Nov 27 17:13:39 EST 1996
In article <Pine.SUN.3.91.961127142201.2572P-100000 at bio02>, Ed
<ed at bio02.bio.uottawa.ca> wrote:
>the frustrating thing is that I can't even hypothesize some massive
>deletion or anything because I have cut out the inserts with enzymes that
>are on opposite ends of the MCS. The primers I'm using for sequencing
>also seem fine because two other clones from the same library screening
>have sequenced beautifully. It's just that the two clones I can't
>sequence are the ones that I would want most dearly for they are the ones
>with longest inserts.
If you can cut out the insert, why not just subclone it into some lovely
fresh new pBluescript/vector of your choice.....
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