how dangerous is ethidium bromide

Paul N Hengen pnh at ncifcrf.gov
Wed Nov 27 16:28:25 EST 1996


Stefano casalotti (stscs at mucc.mahidol.ac.th) wrote:

> I generally put Ethidium bromide directly in the agarose gel but other
> people prefer to stain the gel after, saying that it is safer. It seems to
> me that one uses much lower concentrations  by applying directly in the
> gel, but of course some of it will elute out in to the tank buffer
> What is the safest method

This topic has been covered here many times before. If you want to see
what people think about the hazards, use, and disposal of EtBr, check the
FAQ list, search the archives, and read the TIBS column on the M&R Web Page.
I doubt you'll uncover anything new by asking the question again.

--
*******************************************************************************
* Paul N. Hengen, Ph.D.                           /--------------------------/*
* National Cancer Institute                       |Internet: pnh at ncifcrf.gov |*
* Laboratory of Mathematical Biology              |   Phone: (301) 846-5581  |*
* Frederick Cancer Research and Development Center|     FAX: (301) 846-5598  |*
* Frederick, Maryland 21702-1201 USA              /--------------------------/*
* - - -  Methods FAQ list -> ftp://ftp.ncifcrf.gov/pub/methods/FAQlist - - -  *
* -  TIBS column archive -> http://www-lmmb.ncifcrf.gov/~pnh/readme.html - -  *
* - The BEST Molecular Biology HomePage -> http://www-lmmb.ncifcrf.gov/~pnh/  *
*******************************************************************************



More information about the Methods mailing list