T/A PCR cloning

gbga14 at udcf.gla.ac.uk gbga14 at udcf.gla.ac.uk
Thu Nov 28 08:33:02 EST 1996


Does anyone have a method for adding a single T to the 3'OH of blunt cut 
plasmid for use in A/T Tac generated PCR fragment cloning.  I am working 
on the assumption that dideoxy T and terminal transferase are used, 
however a protocol known to work would be very useful



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