un691cs at genius.embnet.dkfz-heidelberg.de
Thu Nov 28 11:08:44 EST 1996
In article <57jvut$2h1 at mserv1.dl.ac.uk>, RMCMAHON at ollamh.ucd.ie says...
>> > At 09:20 AM 11/23/96 -0600, Rick Bright wrote:
>> > >I have extracted DNA from whole blood and have checked the purity using
>> > >the 260/280 ratio. My professor then asked me how much DNA I extracted,
>> > >in micrograms. Stumped as I was, I thought I would ask for a method or
>> > >equation that would tell me this. A rough estimate is fine, is there is
>> > >an easy technique or formula. Thanks.
>> > Is there a trick question embedded in that? Shouldn't a student
>working with DNA be able to do the calculation without resorting to
>> By taking time to check a book....mmm...he should...
>Could we not keep answers constructive on this group?
>Ruth McMahon, PhD.
Then why don't yah, Ruth ?
Let me try (altho I am doing this without a text book,
....uhm o.k..... Say you have a volume x and an OD260 = y
1 OD unit dsDNA is 20 micrograms per ml, by definition.
Then you would have (y * 20 / x) micrograms. Correct ?
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