Staining agarose gels

Administrador de iihema.fmed.uba.ar postmast at iihema.fmed.uba.ar
Thu Nov 28 21:16:35 EST 1996


josh at panda.uchc.edu (Josh Goldstein) wrote:

>  I have successfully stained gels with acridine orange, which you can
>then visualize with a UV lamp. The advantage is that ssDNA glows red, and
>dsDNA glows green.  For a reference see Gary K. McMaster and Gordon G.
>Carmichael, PNAS vol.74, no.11, pp4835-8, 1977.  Note, however, that you
>need to use 100fold LESS acridine orange than they suggest, or it takes a
>week to destain.

How much would that be?  (sorry, don't have access to PNAS from 1977).
How sensitive is it?


Facundo Garcia Bournissen,
postmast at iihema.fmed.uba.ar



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