pGEM3Zf-

Pascal Mertens Pascal.Mertens at fundp.ac.be
Fri Nov 29 07:51:05 EST 1996


In article (Dans l'article)
<Pine.A32.3.91.961125144804.68358A-100000 at spider.uspnet.usp.br>,
mdamarin at usp.br (Mozart de Azevedo Marins) wrote (écrivait) :

> Hi there,
>          We are using a pGEM derivative plamsid called pGEM3Zf- from 
> promega for sub-cloning some DNA inserts. In the course fo our experiment 
> we have ended up now with one of the clones showing a deletion, and 
> altered pattern in the restriction enzyme map. Since then we have met 
> three other cases of people who have problems with this plasmid. I would 
> like to know if some of you also have these types of problem with this 
> palsmid.
>           Thanks,
>           Mozart 

I received once a gene cloned in this vector: never had problems but I
just used it to subclone in another plasmid.
Pascal

-- 
Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
URBM-FUNDP
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420



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