Help with Lysis Buffer 4 adherent cells please !

runting at wehi.edu.au runting at wehi.edu.au
Fri Nov 29 03:17:39 EST 1996


HI there

I am in need of some suggestions for a new recipe for
lysis buffer to lyse adherent mammalian cells from
96 well tissue culture plates.

The lysis buffer I am using at the moment is 30mM Tris,
100mM EDTA with 20% Sarkosyl. I am getting unreliable
results using this at the moment.

Any suggestions as to a new lysis buffer which might
work better in triated thymidine uptake assays which
I am using would be greatly appreciated

Thanks in advance,

Andrew Runting
Ludwig Institute for Cancer Research
Melbourne Australia
runting at licre.ludwig.edu.au



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