Concentration of DNA

Mart Speek mspeek at tamm.eenet.ee
Sat Nov 30 13:50:39 EST 1996


Paul N Hengen (pnh at ncifcrf.gov) wrote:
: fang at SASK.USASK.CA wrote:
: > Dear netter,
: > How do you measure DNA concentration accurately? By gel (agarose or 
: > acrylamide) or by spectrophotometer? What is the lowest concnetration you 
: > can get? Say the DNA is 500 - 1 kb in size. How do you measure very low 
: > concentration, eg. around 5 ng/ul, and you could not afford 1 ml of DNA 
: > sample for measureing? 

Dear Paul & Li;

In a carefully designed experiment you can check a few nanograms of DNA
>500 bp. This is in TBE-agarose (yields sharper bands), run at high voltage
with defined amounts of DNA markers such as EcoR1 or HindIII Lambda DNA 
digest or similar of any small plasmid. This is good for visual estimation.

Mart

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