How to do NATIVE PAGE?

Thorsten Schmidt Thorsten.Schmidt at rz.ruhr-uni-bochum.de
Sat Nov 30 12:48:47 EST 1996


Dear Reader!

I want to separate my target protein from other proteins in low
concentration
via native PAGE (poly acrylamide gel electrophoresis).
What do I have to think about when planning the experiment?
Where are the differences between native PAGE and SDS-PAGE?

I heart that one has to calculate the isoelectric point.
But how can I do this with a 300 aa-protein? (the sequence is known)

Thank you for your answer!

Thorsten Schmidt




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