Methods November 1996 Archives by author

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Starting: Fri Nov 1 00:34:55 EST 1996
Ending: Sat Nov 30 13:56:23 EST 1996
Messages: 1070

? DNase I degrades dsDNA and/or ssDNA Juergen Sasse (Path)
ds oligo purification Juergen Sasse (Path)
Localisation of CA microsatellites Juergen Sasse (Path)
methylation assay - 3H vs. 14C *
Expression using metallothonein promoter in CHO cells . at dub-mail-svc-1.compuserve.com
transfection of HL-60 . at dub-mail-svc-1.compuserve.com
Transfection of HL-60 . at dub-mail-svc-1.compuserve.com
Biotin labelling of Cell Extracts for Western Blots . at dub-mail-svc-1.compuserve.com
Transfection of HL60 . at dub-mail-svc-1.compuserve.com
TRIzol . at dub-mail-svc-1.compuserve.com
c-Abl expression vector wanted . at dub-mail-svc-1.compuserve.com
REQ: PCR primers - help wanted 9605574b at udcf.gla.ac.uk
Help Culture of QT-6 cells 難波　恒久
Help: Desalting Oligos JAMES ABBOTT
Do you need to remove gene promoter before insertion into an expression vector? JAMES ABBOTT
Transfection & RNA slot blots Mr Hassan Abdulrazzak
Long RT-PCR Amin Abujoub
pcr Amin Abujoub
metabolic pathway charts Achim Recktenwald, PhD
Best way to clean cuvettes? Achim Recktenwald, PhD
looking for protocol dansyl chloride Achim Recktenwald, PhD
imidazole inhibit enzyme activity Achim Recktenwald, PhD
Autoclaving Reagents Achim Recktenwald, PhD
Autoclaving Reagents Achim Recktenwald, PhD
imidazole inhibit enzyme activity Achim Recktenwald, PhD
HELP: Purification of his-tagged protein David Ackerley
Sonic Hedgehog antibodies? Dave Adelson
multiplex PCR Dave Adelson
DNA BY CHELEX 100 EXTRACTION Dr Alok Adholaya
DNA Extraction Protocol Dr Alok Adholaya
BIOSCI/bionet miniFAQ & Fundraiser BIOSCI Administrator
Inclusion of restriction sites ...ERRATUM Ashok Aiyar
Inclusion of restriction sites ...ERRATUM Ashok Aiyar
Inclusion of restriction sites ...ERRATUM Ashok Aiyar
Inclusion of restriction sites ...ERRATUM Ashok Aiyar
yeast two hybrid library Ashok Aiyar
BL21(DE3) pLYSe/s competent cells Ashok Aiyar
KanaR in bacteria=NeoR in eukaryotic cells? Ashok Aiyar
supF selection, pcDNAI-neo Ashok Aiyar
Storm vs Molecular Imager Ashok Aiyar
Thermostable Klenow Ashok Aiyar
differential display RT-PCR Louis Alarcon
How to purify DNA from PAGE? Louis Alarcon
help dd-PCR Louis Alarcon
checking ligations by PCR, help. Louis Alarcon
Can ssDNA transform competent cell ? P.R. Alefounder
BL21(DE3) pLYSe/s competent cells P.R. Alefounder
HL60 transfection thread Danny Altschuler
pH measurements of small biological samples Dan Altura
pH measurements of small biological samples Dan Altura
FREE pH Measurement booklet Dan Altura
FREE pH Measurement booklet Dan Altura
Reasons for PCR failure....? Bruce Amsden
MOLCULAR WEIGHT MARKERS Bruce Amsden
DNA size markers Bruce Amsden
Phagemid-Kit for Kunkel Mutagenesis commercially available ? Chen Ho An
Most Consistent Total RNA Extraction Method/Kit? Eric Anderson
Where is 12CA5? Thomas R. Anderson
estrogen receptor antibody Thomas R. Anderson
quality of Mouse genomic DNA Christophe Andreoli
pcr quality of mouse genomic DNA Christophe Andreoli
Quality of genomic DNA for long PCR Christophe Andreoli
make a hot probe by PCR Christophe Andreoli
HELP: Purification of his-tagged protein Christophe Andreoli
Nonfat Milk Powder & Western Blot Anonymous
Why freeze autorads? Anonymous
Electrophoresis of cholesterol Lo Wing Ip Anthony
pcr Lo Wing Ip Anthony
Autoclaving Reagents Lo Wing Ip Anthony
DNA from Whole Blood Lo Wing Ip Anthony
chromosome 19 mapping Helen Ardley
question about PCR Ahmet Arman
question regarding making SS DNA Ahmet Arman
Nonfat Milk Powder & Western Blot Curt Ashendel
Where is 12CA5? Graham Atherton
IRES elements Oscar Aurelio
Experience retroviral-tet expression systems Oscar Aurelio
Prep of DNA for electroporation BARRETT at RASCAL.MED.HARVARD.EDU
Prep of DNA ... followup BARRETT at RASCAL.MED.HARVARD.EDU
Gycogen co-precipitation with DNA? Joel Bader
unsubscribe Brendan Baggot
(none) Brendan Baggot
Staining agarose gels Joseph C. Bagshaw
ABI 310 Gene Analyzer Joseph C. Bagshaw
Enzyme histochemistry tissue preparation help needed Janice Bailey
Preast Protein gels Banstead at aol.com
Precast protein gels Banstead at aol.com
rifampicine Tim Barnett
purifying dna from gel slices Kirk A. Bartholomew
fluorescence anisotropy Lara Endres Baxley
DNA precipitation without phosphate Olivier Becherel
hagfish mtDNA Andreas Becker
Australian PCR Techniques Workshop Chris Becker
DNA size markers Robin Beech
Campylobacter Gyrase A Robin Beech
Wanted: Cholera toxin B subunit K.T. Beggs
Help to find Methicillin M C Behrendt
source of 35S-labeled anti-rabbit antibodies needed Vadim Beilinson
E.coli strain Michael Benedik
Nonfat Milk Powder & Western Blot Michael Benedik
Reasons for PCR failure....? Gina Berardesco
HPLC solvents: filter-degass Mike Berg
Nonfat Milk Powder & Western Blot Stefan Beyreuther
Inverse PCR Stephen J Billington
GST monoclonal antibody Jian Zhang - Cell Bio
Why freeze autorads? Ransom Hill Bioscience
Library insert DNA Rory O'Brien. Biotechnology.
Purifying DNA from gel slices David Bird
Phenol David Bird
Request for TGFbeta3 Allen Black
Phospholipid Assay Needed Shaun D. Black
E-Mail Virus Warning Shaun D. Black
Wanted: "Good" buffers for pH <5.5 Shaun D. Black
Why freeze autorads? Samuel C. Blackman
RNA purification question !!!!!!! Volker Blaschke
Have you heard of this: In vivo deletions of pCRII Volker Blaschke
Rat lens library Bob
in situ hybridization Roald Boe
S: Labeled ribonucleotide Bolten at ubaclu.unibas.ch
How to wash glassware for using with mRNA experiment Joe Boutell
DNA Quantification Joe Boutell
AmpliTaq Gold Kim Boutilier
Autoclaving Reagents Chris Boyd
E.coli strain Chris Boyd
Electroporation cuvettes Chris Boyd
E.coli strain Chris Boyd
G-HCl and LiCl compatible for RNA prep? Alex Brands
Knock-Out, which vector Alex Brands
Prep of DNA for electroporation John Brennand
DNA Storage Rick Bright
Cell Lysing Detergent Rick Bright
Autoclaving Reagents Rick Bright
Autoclaving: It worked Rick Bright
DNA purity Rick Bright
DNA Quantification Rick Bright
Plant DNA Extraction Rick Bright
good Reverse Transcriptase... Guido Brink
source of wheat germ please Guido Brink
subcloning of large DNA fragments (1,7 Kb) Deb Britt
E.coli strain Thomas Broschard
E.coli strain Thomas Broschard
E.coli strain Thomas Broschard
What are the best GFP tags? Fraser D. Brown
Immunoblot semi-quantitative at best? Jeff Brown
metabolic pathway charts Karen S. Browning
BL21(DE3) pLYSe/s competent cells Paul A Bucciaglia
(none) Ernesto Bustamante
(none) Ernesto Bustamante
Fusion protein activity Dr E. Buxbaum
ECL reagents Dr E. Buxbaum
Home-made fluorometer? Dr E. Buxbaum
Immunoblot semi-quantitative at best? Dr E. Buxbaum
densitometry hardware Dr E. Buxbaum
protein fractionation ? Dr E. Buxbaum
Nonfat Milk Powder & Western Blot Dr E. Buxbaum
GDP Affinity Chromatography Dr E. Buxbaum
acetone precipitation of samples (for SDS PAGEs) Dr E. Buxbaum
coupling with detergent? Dr E. Buxbaum
Need help with Sonicator Dr E. Buxbaum
detergent Dr E. Buxbaum
Bailey's Irish Cream Dr E. Buxbaum
PAGE digestion C.R.Baker
Storm vs Molecular Imager Florence CABON
Human BRCA2 exon1 genomic clone CHAUDH45 at CCVAX.MMC.EDU
Differential Display kit - Display Systems CISCA at kan.rpa.cs.nsw.gov.au
PFGE with LMP? STACY A CIUFO
in situ PCR course Diego Cantalapiedra
Looking For Murine Ubiquitin DNA Gregory B. Carey
bromo UTP Carolyn
DNA-affinity chromatography with AB1 Jose Luis Carrasco
RT-PCR leak? J. Carvajal
lysozyme sources Leslie Carvalho
DNA Quantification Edmundo Castro
DNA Quantification Edmundo Castro
Electroporation cuvettes Cristina Cebrian
cfp: RECOVERIES '97 Petr Cejchan
Preparation of mitotic HeLa cells? Zhonglin Chai
Monkey RNA Angel Raya Chamorro
Reasons for PCR failure....? Brenda Chan
Green fluorescent protein vector Alex Chang
GST vector expression of ScFv Alex Chang
Help:Rat embryo fibroblasts and transformation Jun Chang
Library insert DNA Tom Chappell
pcr quality of mouse genomic DNA Mic Chaudoir
Electropoation of lympocytes and liver cells Philip Ta-Chih Cheng
Long Ranger Acrylamide Casey Chin
Promega fmol seq.kit: Using an alternate Taq Pol? Tim Chipman
Prep of DNA for electroporation Christophe
DNA precipitation without phosphate Christophe
betagal fusion in B. subtilis Christophe
RNA extraction in B. subtilis Christophe
in situ hybridization Shi-Jye Chu
Licensing your thermocycler for PCR? Dr. Duncan Clark
Restriction enzyme SwaI ?? Dr. Duncan Clark
BL21(DE3) pLYSe/s competent cells Dr. Duncan Clark
BL21(DE3) pLYSe/s competent cells Dr. Duncan Clark
long ranger acrylamide Dr. Duncan Clark
Cell Lysing Detergent Dr. Duncan Clark
Bovine Serum Albumin in PCR Dr. Duncan Clark
Prep of DNA for electroporation Dr. Duncan Clark
Autoclaving Reagents Dr. Duncan Clark
Need help with Sonicator Dr. Duncan Clark
Help: Desalting Oligos Dr. Duncan Clark
T/A cloning of PCR products Dr. Duncan Clark
Construction of pMC9 as in Y1088, Y1089, Y1090 Dr. Duncan Clark
2 min/Kb for Pfu polymerase Dr. Duncan Clark
Thermostable Klenow Dr. Duncan Clark
Library insert DNA Dr. Duncan Clark
DNA Quantification Dr. Duncan Clark
Photogene with plaque blots Claudia
purifying Parvovirus B19 DNA from plasma Dr. J.P. Clewley
Inclusion of restriction sites ...ERRATUM Michael Coady
Why freeze autorads? Andrew Cockburn
transcription? Steven Cohen
IRES Experience! Steven Cohen
Potassium Phosphate Buffer Jeff Cohlberg
best mutagenesis kit? Jeff Cohlberg
acetone precipitation of samples (for SDS PAGEs) Jeff Cohlberg
hepatitis B virus? Robin Colgrove
Nonfat Milk Powder & Western Blot Robin Colgrove
Help: Desalting Oligos The Great American Gene Company
Why freeze autorads? The Great American Gene Company
DNA Quantification The Great American Gene Company
Primer deletion The Great American Gene Company
QC Sampling Plan Quality Control
Purifying DNA from gel slices Peter S. Cooper
Wanted:Method for transfection of primary human fibroblasts Dan Coovert
Purifying small qtys of mRNA from neural tissue Marc Cortese
Purifying small qtys of mRNA from neural tissue Marc Cortese
Yeast Extracts Maria C. Costanzo
BL21(DE3) pLYSe/s competent cells Francois Coulier
Isolating RNA without using DNase?? Francois Coulier
Purifying DNA from gel slices Francois Coulier
WHY DON'T WE FREEZE 35-S AUTORADS ? Francois Coulier
Why freeze autorads? Robert Cowherd
Cell cultures. Requesting information Andrea Crampton
Promoter - probe vector needed Dr Ron Croy
Ubuquitin markers? Jim Cummins
unsubscribe Jason Cyr
His Tag Expression Vector Peter Czabotar
GuCl in SDS Page D.Lippert
Wanted: "Good" buffers for pH <5.5 D.Lippert
KanaR in bacteria=NeoR in eukaryotic cells? Eric DELABESSE
Why freeze autorads? Stephen C. Dahl
Immunoblot semi-quantitative at best? Frank Daniels
Where is 12CA5? David L. Haviland, Ph.D.
Wanted - Insect Genomic Library Mary Beth Davis
GDP Affinity Chromatography Amy DeRocher
New Job Listings at EXPMED JOB LISTINGS (from McGill Div. of EXP. Medicine) Graham Dellaire
VOTE NOW!!! GenStructure news group: bionet.genome.gene-structure. Graham Dellaire
hoechst 33258 and 33342 Jean Philippe Demaret
Reasons for PCR failure....? David Demezas
RNA storage Dennis
Cell Lysing Detergent Paul Digard
National Biotech Register Property Digest
differential display RT-PCR Alexis Diwa
What's up with Afl II and Bfr I? John Dixon
DNA Storage Andrew Doherty
pcr quality of mouse genomic DNA Andrew Doherty
Fusion protein activity Andrew Doherty
attachment HEK-293 cells Andrew Doherty
question about PCR Andrew Doherty
Purifying DNA from gel slices Andrew Doherty
Efficiency of making ssDNA by asymmetric PCR? Andrew Doherty
Inverse PCR Andrew Doherty
Inverse PCR Andrew Doherty
Transfecting LTK cells Andrew Doherty
purifying dna from gel slices Andrew Doherty
TE Buffer for RNA Andrew Doherty
QT6 cells??? Qunfeng Dong
Isolating RNA without using DNase?? Qunfeng Dong
Phenol Gary Dossantos
Acetyl CoA for CAT Harold Drabkin
Chloramphenicol resin Harold Drabkin
Chloramphenicol-sepharose resin Harold Drabkin
WHY DON'T WE FREEZE 35-S AUTORADS ? Tom Duncan
(none) EHEALY at MACOLLAMH.UCD.IE
acetone precipitation of samples (for SDS PAGEs) Doug Easton
baffling pBluescript stuff... Ed
Amphotericin B in plant tissue culture? Adrian Elliott
mRNA Transcript Quantitation Method [Q] Øivind Enger
Where is 12CA5? Hernan Espinoza
acetone precipitation of samples (for SDS PAGEs) Hernan Espinoza
Acid Phosphatase Ab C. FJETLAND
long ranger acrylamide Core Facility
tip racks for Biomek1000 Core Facility
Purifying DNA from gel slices Frank O. Fackelmayer
fluorescent RNA stain for microscopy Frank O. Fackelmayer
GuCl in SDS Page Frank O. Fackelmayer
fluorescent RNA stain for microscopy Frank O. Fackelmayer
Help: Fat seperation Hamilton High Faculty
dansyl chloride protocol Patrizia Fanara
Looking for dansyl chloride protocol Patrizia Fanara
looking for protocol dansyl chloride Patrizia Fanara
Reducing potential of thiol compunds Steve Festin
Riboprobes, Northerns, and high background Karl Fischer
Protocol- hybridization with oligonucleotides Johanne Fleurent
mRNA prep with Promega Kit Noel Fong
TE Buffer for RNA Franek, Karl - Microbiology
Source for Nonidet P-40 (was: Cell Lysing Detergent) Frankster
Prep of DNA for electroporation Frankster
Transfecting LTK cells Frankster
ds oligo purification Bryant Fujimoto
coupling with detergent? Jiro Fujimoto
Western blot project Sarah E Furey
DNA precipitation Masato Furuichi
SCIENCE PROJECT FOR 7th GRADE ANNE GEORGE
Why freeze autorads? THE GREEK
Why freeze autorads? Warren Gallin
Why freeze autorads? Warren Gallin
intronless gene in mammary gland? Olivier Gandrillon
E-Mail Virus Warning IS AN HOAX Olivier Gandrillon
Best Long PCR? Dan Gebow
Why freeze autorads? Dr. Peter Gegenheimer
Why freeze autorads? Dr. Peter Gegenheimer
DNA/RNA purification R. Dana - Green Gene
Request for phage display library Sourav Ghosh
Riboprobes, Northerns, and high background Gil
Cloning Unstable Sequences Jon Gilthorpe
S6 protein anyone ? Ole Gjoerup
hot PCR probe? Michelle Gleeson
LambdaGEM-11 vector - Kpn sites Michelle Gleeson
Precast Protein Gel Prices GlobalEngr at aol.com
Two PCR quesions Steven Goldberg
BAC vectors/libraries Steven Goldberg
In situ PCR of bacterial colonies? Steven Goldberg
Have you heard of this: In vivo deletions of pCRII Steven Goldberg
Wanted: "Good" buffers for pH <5.5 Hugh Goldie
DNA purity Josh Goldstein
Staining agarose gels Josh Goldstein
hepatitis B virus? Jose Gonzales
Insoluble GST-fusion Ian Goodfellow
Prep of DNA for electroporation Martin Goodson
QuikChange Richard P Grant
Electroporation cuvettes Richard P Grant
Help: Desalting Oligos Richard P Grant
Microplasma Richard P Grant
lambda gt11 fibroblast library Catherine Greene
DNA BY CHELEX 100 EXTRACTION Steve Griffiths
Magnetic Protein-A Keith Grimaldi
interference of Qaiex with biotinylated primers???? Peter J van Coeverden de Groot
Gycogen co-precipitation with DNA? Kathie Grovit-Ferbas
WANTED: Marking Pen for Autorads Mike Gruidl
Need help with Sonicator Toumy Guettouche
Autoclaving Reagents Toumy Guettouche
Nonfat Milk Powder & Western Blot Toumy Guettouche
Why freeze autorads? Toumy Guettouche
pBluescript mcs addition David Guttman
supF selection, pcDNAI-neo Lemay Guy
peptide quantitation HDZ
tagetitoxin and amanitin Mary Kay Hamm
constitutive probes Neil Harris
constitutive probes Neil Harris
GuCl in SDS Page Jared Head
Autoclaving Reagents Anssi Heikkilä
nitrate reductase -sweet potato Yair M. Heimer
Nonfat Milk Powder & Western Blot Alix Helme-Guizon
rna removal from plasmid preps with Li Cl precipitation Nick C.K. Heng
Tracking Dye Paul N Hengen
Pointer to FAQ lists Paul N Hengen
ds oligo purification Paul N Hengen
purifying dna from gel slices Paul N Hengen
Purifying DNA from gel slices Paul N Hengen
chloramphenicol resin Paul N Hengen
antisense technology? Paul N Hengen
Why freeze autorads? Paul N Hengen
antisense technology? Paul N Hengen
Screening of ESTs with cDNA probe Paul N Hengen
Concentration of DNA Paul N Hengen
Nonfat Milk Powder & Western Blot Paul N Hengen
Staining agarose gels Paul N Hengen
Gycogen co-precipitation with DNA? Paul N Hengen
DNA Quantification Paul N Hengen
Bailey's Irish Cream Paul N Hengen
Bailey's Irish Cream Paul N Hengen
how dangerous is ethidium bromide Paul N Hengen
purifying Parvovirus B19 DNA from plasma D. Herns
Sonic Hedgehog antibodies? Phil Hertzler
YACs David Hills
her-2/neu David Hills
Just try this, it will work Hung-yiu Ho
Please stop any advertisement in this newsgroup Hung-yiu Ho
Bailey's Irish Cream Louis Hom
request Microfluidizer and Zeocin Ouyang Hongsheng
who sale the anti E. Coli. 0157 sera and anti E. Coli H7 sera Ouyang Hongsheng
How to purify DNA from PAGE? Guido Hooiveld
DNA BY CHELEX 100 EXTRACTION Jan Hoolwerf
Help needed with PCR of eggs Hopkins, Charlotte
RT-PCR leak? Ken Howe
Functional Genomics!! Ken Howe
Genome databases: strengths and weaknesses Ken Howe
help for Tsp4cI Q. Hu
reversible disruption of nuclear membranes? Chris Hughes
cDna screening Jim Hughes
PCR on mouse genomic DNA from blood Simon Hughes
Why freeze autorads? Eric R. Hugo
c-Abl expression vector wanted Jean-Karim Hériché
Have you heard of this: In vivo deletions of pCRII Jean-Karim Hériché
checking ligations by PCR, help. Carsten Höge
AmpliTaq Gold ImmGen, Inc.
DNA from Whole Blood Mike Irwin
TMAC in Southern blots Ramaswamy K. Iyer
checking ligations by PCR, help. Fraser Dr N J
Insoluble GST-fusion JL at DNA.BIO.WARWICK.AC.UK
differential display JUAN
Branch Chain Amplification JWilk11912
question about PCR Nick Jacobsen
electroporation and ion-channel currents Nick Jacobsen
Giemsa staining after Drosophila polytene chromosome in situ hybridization Thomas Jaw
Giemsa staining after Drosophila polytene chromosome in situ hybridization Thomas Jaw
Sequence of pCNB5 [Help!] Je, Hyun-Soo
Source for Nonidet P-40 (was: Cell Lysing Detergent) Jenni
RNA purification question !!!!!!! Teis Jensen
Deletion analysis Changcun Jia
DD-PCR shou Jiang
Specificity of DNAseI in Mn++ Douglas Johnson
ECL reagents P. Jones
T. aquaticus/thermophilus genomic libraries? Luca Jovine
Inverse PCR Cristina Juarez
Non-secreted Human Alkaline phosphatase Diane Judd
B or T cell cDNA banks Alan KAY
Insoluble GST-Fusion Protein KHUANG at CHEMVX.TAMU.EDU
Site-directed mutagenesis KHUANG at CHEMVX.TAMU.EDU
Fusion protein ativity KHUANG at CHEMVX.TAMU.EDU
Recovery protein from CBB stained SDS-PAGE KHUANG at CHEMVX.TAMU.EDU
Suggestions for expresssion two proteins in E. coli KHUANG at CHEMVX.TAMU.EDU
subclone large fragment KHUANG at CHEMVX.TAMU.EDU
imidazole inhibit enzyme activity KHUANG at CHEMVX.TAMU.EDU
Imidazol inhibit enzyme activity KHUANG at CHEMVX.TAMU.EDU
2 min/Kb for Pfu polymerase KHUANG at CHEMVX.TAMU.EDU
metabolic pathway charts Chris Kafer
live cells reduced J Kahl
estrogen receptor antibody: which do you reco. Stefan Kahlert
Staining agarose gels Makoto Kamei
baffling pBluescript stuff... Makoto Kamei
Northern blot trouble Fredrik Kamme
Problem with RACE Priya Kannan
Codon preferences Jill Kellogg
Trizol Nigel Kenward
Bailey's Irish Cream Nigel Kenward
Rna gel Nigel Kenward
PhD Position availabl Gabriele Kerber
Vogel-Bonner? Michael Kertesz
Bovine Serum Albumin in PCR Michael Kertesz
ganciclovir Shirin Khambata
pCMV-2-FLAG vector sequence Myung Jong Kim
Where to send proteins for N-terminal aa sequencing? Michael R. King
imidazole inhibit enzyme activity KingO'D'alasis at tamu.edu
IRES Experience! Csaba Kiss
protein fractionation ? Svend Kjaer
FREE pH measurement booklet R. Klein
Immunoblot semi-quantitative at best? Dima Klenchin
GST monoclonal antibody Dima Klenchin
Prep of DNA for electroporation Dima Klenchin
Wanted:Method for transfection of primary human fibroblasts Dima Klenchin
Method: Electroporate COS-7 Dima Klenchin
Nonfat Milk Powder & Western Blot Dima Klenchin
How to show protein interaction? Dima Klenchin
question regarding making SS DNA Bob Kodrzycki
CMV - driven marker for stable expression Riekeltje Koedood
Reasons for PCR failure....? Riekeltje Koedood
E-Mail Virus Warning Roland Koelliker
Free kit for determination of hyaluronan! Prachya Kongtawelert
Tissue culture antibiotics John Korte
GST vector expression of ScFv Peter B. Kos
AmpliTaq Gold Alexander Kraev
AmpliTaq Gold Alexander Kraev
Why freeze autorads? Cornelius Krasel
Column packing problem Rajesh Krishnamurthy
rifampicine Georg Kroeger
HELP! Gel analyzing software Georg Kroeger
DNA from Whole Blood Jerry Kropp
re half-life of biotinylated primers Jerry Kropp
Thermostable Klenow Jerry Kropp
Purifying DNA from gel slices Valeri Krougliak
long ranger acrylamide Gary Kucera
pcr quality of mouse genomic DNA Gary Kucera
Riboprobes, Northerns, and high background Gary Kucera
Nuclear extracts/suspensions Alexander N. Kukushkin
Wanted : protocol for bandshifts Alexander N. Kukushkin
Purifying DNA from gel slices Alexander N. Kukushkin
bcl-2 gene structure Alexander N. Kukushkin
Two PCR quesions Rajesh Kumar
Where is 12CA5? Tony Kwan
Home Brew-Genescan Standards GARRY LARSON
rRNA? SHAWN EDWARD LUPOLD
BL21(DE3) pLYSe/s competent cells Rico Laage
best mutagenesis kit? Rico Laage
reprobing NBT/BCIP-Westerns with ECL? Rico Laage
Kin28 antibody Lis Lab9
pcr quality of mouse genomic DNA John Ladasky
T/A cloning of PCR products Kevin J. Laddison
RNA storage Eric Lader
Molbio-Software (Macintosh) Bernd Langkau
Stratagene QuikChange--tandem inserts!!! Tim Lansing
Problems with D5S392 Flemming Larsen
Restriction enzyme SwaI ?? Michael Lassner
rTth reverse transcriptase kit Michael Lassner
Help: Desalting Oligos Michael Lassner
plasmid library amplification Franck Laurent
Isolating RNA without using DNase?? Edouard Lauzier
DNA Quantification Edouard Lauzier
cloning problem (pGL2basic, pGL3basic) Philippe Lefebvre
best mutagenesis kit? Philippe Lefebvre
PAGE digestion Philippe Lefebvre
Q: pGL3basic: Ligation TIME and TEMPERATURE?? Philippe Lefebvre
GuCl in SDS Page Philippe Lefebvre
WHY DON'T WE FREEZE 35-S AUTORADS ? Philippe Lefebvre
2.4 kb PCR Rachelle Leger
pH Measurement of Small Biological Samples Steven Lemore
attachment HEK-293 cells Rob Leurs
acetone precipitation of samples (for SDS PAGEs) Wulf Dirk Leuschner
RNA:RNA in situ hybridization Lonny R. Levin
help: need a way to stain Gall Bladder of Xenopus embryos Michael Levin
need a protocol to do a Western blot for membrane protein from Xenopus Michael Levin
Baculovirus expression system David N. Levy
What's up with Afl II and Bfr I? Mark Leyland
Competitive blocker pcr Kimberly Lieuallen
TUNEL labeling of fixed tissue: Carnoy's vs paraformaldehyde Richard Lightfoot
Purifying DNA from gel slices Nancy Linford
Spectrophotometer for kinetics analysis Ji-quan Liu
acetone precipitation of samples (for SDS PAGEs) David C. Logan
Concentration of DNA David C. Logan
hagfish mtDNA J. Andres Lopez
Desalination Martin Luetzelberger
lysozyme sources Minh Luu
Site-directed mutagenesis Lynne_M_Roxbee_Cox
intronless gene in mammary gland? RUTH MCMAHON
DNA Quantification RUTH MCMAHON
Help Making DNA <100bp MELANSOND
T/A cloning of PCR products MELANSOND
Hitachi U-2000 Data output FABIAN MOEBIUS
genomic sequencing Sean MacAteer
Magnetic Protein-A Ian MacCoubrey
DNA Quantification Dr. Michael T. MacDonell
T/A cloning of PCR products John MacDougall
pcr quality of mouse genomic DNA Ulrich Maier
E-Mail Virus Warning Rafael Maldonado
Inverse PCR Shehrebanoo Malik
2.4 kb PCR Shehrebanoo Malik
2.4 kb PCR Shehrebanoo Malik
In situ PCR of bacterial colonies? Shehrebanoo Malik
WWWtacg Version 1.6 - Restriction Enzyme Analysis via the Web Harry Mangalam
mRNA Transcript Quantitation Method [Q] Ned Mantei
Specificity of DNAseI in Mn++ Ned Mantei
yeast two hybrid library Jeremy Marcus
Why freeze autorads? Jonathan B. Marder
Why freeze autorads? Jonathan B. Marder
Codons Mozart de Azevedo Marins
pGEM3Zf- Mozart de Azevedo Marins
Drosophila DNA transf. Mozart de Azevedo Marins
Hydroxylamine protocol Mark Brandt, Ph.D.
long ranger acrylamide Steve Marsh
Biotin labelling of Cell Extracts for Western Blots Dr. David Martin
Preast Protein gels Dr. David Martin
coupling proteins to CNBr activated sepharose... Dr. David Martin
Bailey's Irish Cream Dr. David Martin
RNA storage Juan Pablo Martinez-Soriano
FACS Roz McDonnell
WANTED: Marking Pen for Autorads Donald G. McEwen
Storm vs Molecular Imager Todd McGee
Cell Lysing Detergent Fergus R. McKenzie
Alk. phosphatase substrates Robert Means
Hydroxylamine protocol Robert Means
Thermal Cycler Purchase Richard K. Meister
Cloning Unstable Sequences Jose E. Mejia
Staining agarose gels Pascal Mertens
pGEM3Zf- Pascal Mertens
Methanol Fixation Alex Merz
Methanol Fixation Alex Merz
Inclusion of restriction sites in PCR primers Alex Merz
Inclusion of restriction sites in PCR primers Alex Merz
Alk. phosphatase substrates Alex Merz
pGL3 is terrible!!! Joe Miano
Reverse Northern? Joe Miano
chicken genomic library? Joe Miano
Bromophenol Blue and PCR? Joe Miano
BAC Transgenics? Joe Miano
Functional Genomics!! Joe Miano
metabolic pathway charts Ted Michelini
Inverse PCR Ted Michelini
T. aquaticus/thermophilus genomic libraries? Ted Michelini
Inverse PCR and RB primers Ted Michelini
AmpliTaq Gold Ted Michelini
Thermostable Klenow Ted Michelini
Riboprobes, Northerns, and high background Ted Michelini
Inclusion of restriction sites ...ERRATUM David Micklem
Inclusion of restriction sites ...ERRATUM David Micklem
Concentration of DNA David Micklem
Bailey's Irish Cream David Micklem
baffling pBluescript stuff... David Micklem
Precast protein gels Jim Miller
Touchdown PCR during Recombinant PCR? Barry Mook
interference of Qaiex with biotinylated primers???? Barry Mook
Doxycycline stable in H2O? Barry Mook
subcloning of large DNA fragments (1,7 Kb) Barry Mook
Bailey's Irish Cream Michael Morales
Xenopus genomic DNA D.D. Morgan
Microplasma Mr. G. Morley
RNA purification question !!!!!!! Thibaud Le Mouel
Rna gel Thibaud Le Mouel
RNA storage Kevin Mulcahy
Nuclear extracts/suspensions Kevin Mulcahy
Nuclear extracts/suspensions Kevin Mulcahy
Nuclear extracts/suspensions Kevin Mulcahy
Nuclear extracts/suspensions Kevin Mulcahy
Nuclear extracts/suspensions Kevin Mulcahy
Nuclear extracts/suspensions Kevin Mulcahy
Re-using electroporation cuvettes Kevin Mulcahy
Electroporaton cuvettes - can you recycle them? Kevin Mulcahy
Vector pHebo: Mobilisation ability? Kevin Mulcahy
Electroporation cuvettes Kevin Mulcahy
Electroporation cuvettes Kevin Mulcahy
Do you need to remove gene promoter before insertion into an expression vector? Kevin Mulcahy
Recovery from liq nitrogen of transfected-LCL? Kevin Mulcahy
Effect of TE on ligations? Kevin Mulcahy
Alk. phosphatase substrates Gordon Munro
Purifying DNA from gel slices Gordon Munro
Size of Taq Pol and klenow Gordon Munro
Indirect Tyramide amplifications Gordon Munro
bromo UTP Frank Murphy
Wanted: Method for transfection of primary human fibroblasts Bernard Murray
HELP: Purification of his-tagged protein Daniel Mytelka
slippage N.Hardman
Nonfat Milk Powder & Western Blot Jon Nakamoto
rna removal from plasmid preps with Li Cl precipitation Your Personal Name
RNA extraction Mr. E.W.A. Needham
WANTED: Marking Pen for Autorads Jacalyn S. Newman
Reasons for PCR failure....? Jacalyn S. Newman
test Lyle Noordhoek
test Lyle Noordhoek
elution of oligo probes from PA gel Pamela Norton
Accuracy of Reverse Transcriptase-Derived Sequences Pamela Norton
DNA Quantification Thomas J. O'Neill
sequence analysis on commercial basis OFFRINGA at rulsfb.LeidenUniv.nl
mRNA Transcript Quantitation Method [Q] Michael Oda
Most Consistent Total RNA Extraction Method/Kit? Michael Oda
Silver Staining for Proteins Martin Offterdinger
rTth reverse transcriptase kit Martin Offterdinger
Wanted : protocol for bandshifts Martin Offterdinger
Biotin labelling of Cell Extracts for Western Blots Martin Offterdinger
Biotin labelling of Cell Extracts for Western Blots Martin Offterdinger
Long RT-PCR Martin Offterdinger
Cell cultures. Requesting information Martin Offterdinger
Sequence analysis??? Takbum Ohn
Why don't my plasmid freeze in freezer? Takbum Ohn
BioOven cycler Elizabeth E Ojeda
Imidazole Info The Kindly One
[EtBr] measurements???? The Kindly One
"APOPTOSIS Online" Site Announcement APOPTOSIS Online
Subcloning of PCR product containing 7-deazoguanine. Sergey V. Orlov
DNA Quantification Otter
pPoly III polylinker ROSEMARY KANE (PHARMACOLOGY) PG
RNA GELS DEREK POTTER
secretory proteins Gurcharan Pahwa
secretory proteins Gurcharan Pahwa
Look here! Pan Parag
Screening of ESTs with cDNA probe Joon-Hyun Park
Reverse Northern? Julian Parkhill
Codon preferences Derek Parsonage
RNA extraction in B. subtilis Derek Parsonage
Immunoblot semi-quantitative at best? Mick Partis
Purifying DNA from gel slices Chris Partridge
knock-outs Chris Paszty
HELP LYSOZYME Fabio Perciaccante
In vivo Protease Inhibitors Dr. Michael Perry
Best way to clean cuvettes? Pete
steraloids Pete
cloning problem (pGL2basic/pGL3basic) Jennifer Philhower
KanaR in bacteria=NeoR in eukaryotic cells? Andy Phillips
ds oligo purification Andy Phillips
rifampicine C E Pical
ECL reagents A. Piccin
Neutrophil isolation Eva Pietrzak
Peptide analysis through proteolysis Pineda
Inclusion of restriction sites in PCR primers Andrei Popov
Inclusion of restriction sites ...ERRATUM Andrei Popov
Inclusion of restriction sites ...ERRATUM Andrei Popov
Inclusion of restriction sites ...ERRATUM Andrei Popov
PFGE with LMP? Andrei Popov
Cloning Unstable Sequences Andrei Popov
YACs Andrei Popov
Why freeze autorads? William P Prichett
Bromophenol Blue and PCR? Michael J. Prigge
Help: Desalting Oligos Kiley R. Prilliman
ciprofloxacin and mycoplasma Kiley R. Prilliman
Fluorescent label for starch Kevin Pyke
Purifying DNA from gel slices QIAGEN
pcr quality of mouse genomic DNA QIAGEN
Imidazole Info QIAGEN
Need help in expression of GnRH receptor in insect cells Steve Qi
Methicillin availability ? R.C.Ogden
Protein Phophatases. SARAH LOUISE RAWLINGS
E-Mail Virus Warning Christian Radauer
Inverse PCR Christian Radauer
chloramphenicol resin Uttam Rajbhandary
PCR preferential amplification, question Ana Rakitin
long ranger acrylamide Karthi Ramachandran
Autoclaving Reagents Karthi Ramachandran
M13K07 helper phage: miniplasmids??? Arfaan Rampersaud
Phagemid-Kit for Kunkel Mutagenesis commercially available ? Colin Rasmussen
Staining agarose gels Colin Rasmussen
Purifying DNA from gel slices Colin Rasmussen
GST monoclonal antibody Colin Rasmussen
Purifying DNA from gel slices Alison Reavley
Search for rRNA probes Harold Rees
radioactive probe counter? Harold Rees
daunorubicin binding Johan Renes
densitometry hardware Gordon Rewcastle
Stratagene QuikChange--tandem inserts!!! William Rice
Type I & II membrane proteins? John H Richburg
prokaryotic ribosomal subunits purification Rikke
protein extraction cell culture Everardo Gonzalez Rodriguez
Antibody to 8-OH-2-deoxyguanine Raymond P. Roos
IRES elements Raymond P. Roos
DNA from Whole Blood Steve Rose
antisense technology? Elizabeth Rosenberg
antisense technology? Elizabeth Rosenberg
h-ras specific antibodies ? Steffen Roth
How to purify DNA from PAGE? Rover
RNA storage Hiranya Roychowdhury
RNA storage Hiranya Roychowdhury
Isolating RNA without using DNase?? Hiranya Roychowdhury
Purifying DNA from gel slices Hiranya Roychowdhury
Isolating RNA without using DNase?? Hiranya Roychowdhury
Automated Sequencer: Q? Hiranya Roychowdhury
RNA purification question !!!!!!! Hiranya Roychowdhury
DNA Quantification Hiranya Roychowdhury
DNA Quantification Hiranya Roychowdhury
DNA Quantification Hiranya Roychowdhury
TE Buffer for RNA Hiranya Roychowdhury
Bailey's Irish Cream Hiranya Roychowdhury
sequence analysis program in www Doug-Young Ryu
experience with pMAMneo-LUC Manuel Maria SIMON
experience with pMAMneo-LUC Manuel Maria SIMON
experience with pMAMneo-LUC Manuel Maria SIMON
Phenol extraction problem/phases not separating MARLENE SNYDER
co-expression in mammalian cell lines DAVID PAGE (PHARMACOLOGY) STAFF
pJAM DAVID PAGE (PHARMACOLOGY) STAFF
2 min/Kb for Pfu polymerase Namgoong SUk
acetone precipitation of samples (for SDS PAGEs) Sai
coupling proteins to CNBr activated sepharose... Sai
How to show protein interaction? Sai
acetone precipitation of samples (for SDS PAGEs) Jesus Sanz
UV mutation of PCR products? Jasper Saris
E-Mail Virus Warning Dean H. Saxe
cloning problem (pGL2basic/pGL3basic) Heinz-Juergen Schaefers
cloning problem (pGL2basic, pGL3basic) Heinz-Juergen Schaefers
cloning problem (pGL2basic/pGL3basic) Heinz-Juergen Schaefers
Q: pGL3basic: Ligation TIME and TEMPERATURE?? Heinz-Juergen Schaefers
Antibodies to transcription factors Heinz-Juergen Schaefers
?? Kinetics Software - Post Charlie Schick
How to show protein interaction? Thorsten Schmidt
How to do NATIVE PAGE? Thorsten Schmidt
Difficulties Obtaining Genomic DNA Robert A. Schoenfeld
electroporation and ion-channel currents Bernhard Schu
haptoglobin genotyping Manfred Schuermann
orosomucoid genotyping Manfred Schuermann
ECL reagents Jeff "Newt" Sekelsky
differential display RT-PCR Joachim Seybold
(none) Andrew Shelling
(none) Ao Shi-zhou
Help! Any information about CEPH? Ao Shizhou
transformation/conjugation in Xanthomonas oryzae Francisco Goes da Silva
metabolic pathway charts S. Silverman
Why freeze autorads? S. Silverman
WHY DON'T WE FREEZE 35-S AUTORADS ? S. Silverman
secretory protein Jaswant Singh
secretory protein Jaswant Singh
diatom dna extraction H.J. Sluiman
Stratagene QuikChange--tandem inserts!!! Dave Smith
E.coli strain Dave Smith
Immobilisation of DNA Lee Smith
Electroporation cuvettes Kimberley Snowden
Accuracy of Reverse Transcriptase-Derived Sequences Ken Soderstrom
ECL reagents Vincent Solomon
Home-made fluorometer? Keld Sorensen
WANTED: Marking Pen for Autorads Keld Sorensen
Nonfat Milk Powder & Western Blot Keld Sorensen
Bailey's Irish Cream Augustine Souza
antisense technology? Mart Speek
Concentration of DNA Mart Speek
BL21(DE3) pLYSe/s competent cells Ralf Spenneberg
RNA sequencing Otello Stampacchia
PCR using yeast colonies-info wanted John Stebbins
Suggestions for expressing two proteins in E. coli Boris Steipe
GST vector expression of ScFv Boris Steipe
How to show protein interaction? Boris Steipe
EtBr Gel documentation systems Gordon Stott
Lambda MAX1 Michael Strehle
DNA probe for Kb Ruey-Chyi Su
CpG island trapping protocols Sara Sunden
Perkin Elmer-ABI PCR Machine Blues C. Suter-Crazzolara
1997 Cambridge Symposia Conferences Cambridge Symposia
request Microfluidizer and Zeocin MISONOU Taku
hot PCR probe? Seng-Lai Tan
Cell Lysing Detergent Ron Tate
Fusion protein activity Ron Tate
Bailey's Irish Cream Dr G R Taylor
Suggestions for Baculovirus expression Jeffrey Shane Temple
WANTED: Marking Pen for Autorads Chromosome Terror
Why don't my plasmid freeze in freezer? Chromosome Terror
how dangerous is ethidium bromide Chromosome Terror
Hybridization of colony lifts Michael W. Thompson
E-Mail Virus Warning Michael W. Thompson
Northern blot Rosemary Thresher
Cell Lysing Detergent Laurence Tiley
Favorite expression systems michael Tilley
Campylobacter Gyrase A Tim
Why freeze autorads? William Tivol
in situ hybridization Dr. A. Torchinsky
RNA storage Mathew Traini
Nuclear extracts/suspensions Mathew Traini
RNA storage Mathew Traini
Nuclear extracts/suspensions Mathew Traini
GDP Affinity Chromatography Mathew Traini
32P-labelling of a peptide Jean-Philip Truman
Purifying DNA from gel slices Chung Jui Tsai
Antibodies to transcription factors Jonathan Tugwood
Desalination Jonathan Tugwood
cDNA Ligation Cleaning Jonny Tuna
Seeking RNA assay Bradley Turner
Yeast 2-hybrid artifacts Przemko Tylzanowski
ABI 310 Gene Analyzer Przemko Tylzanowski
Source for Nonidet P-40 (was: Cell Lysing Detergent) Darren Tyson
densitometry hardware Darren Tyson
Immunoblot semi-quantitative at best? Darren Tyson
How to wash glassware for using with mRNA experiment Panapat Uawithya
test Makoto Uchino
detergent Makoto Uchino
purifying dna from gel slices Paul Ulanch
tagetitoxin and amanitin Paul Ulanch
Cloning Unstable Sequences Edgar Valencia
pGEX-1 Nataraj Vettakkorumakankav
pGEX-1 Nataraj Vettakkorumakankav
Phagemid-Kit for Kunkel Mutagenesis commercially available ? Tom Vink
Pigments are coextracted with RNA.... Savita Visal
Promoter - probe vector needed Dr A.H.M. Van Vliet
GuCl in SDS Page Dr A.H.M. Van Vliet
Baculovirus expression system DAVID R. WESSNER
Help Making DNA <100bp WSchick at aol.com
long ranger acrylamide WSchick at aol.com
Fwd: DD-PCR WSchick at aol.com
request Microfluidizer WSchick at aol.com
AmpliTaq Gold WSchick at aol.com
wanted: tet resitance casette Robert R Wagner
Western blot project Nigel Walker
multi-bands on microsatellites Shelley Walton
Can ssDNA transform competent cell ? Peter Wang
Antibodies to E. coli DNA polymerase? Peter Wang
Efficiency of making ssDNA by asymmetric PCR? Peter Wang
Inverse PCR and RB primers Xiao-Hui Wang
Cell Lysing Detergent John Watson
Potassium Phosphate Buffer John Watson
Re-using electroporation cuvettes John Watson
How to purify DNA from PAGE? John Watson
Autoclaving Reagents John Watson
KlentaqLA extra A Wayne M. Barnes, Ph.D.
rna removal from plasmid preps with Li Cl precipitation Lee Weber
Type I & II membrane proteins? Gregg B Wells
acetone precipitation of samples (for SDS PAGEs) Chi-kuang Wen
Prep of DNA for electroporation Chi-kuang Wen
mRNA prep with Promega Kit Chi-kuang Wen
Storm vs Molecular Imager P. Willemsen
QuikChange Tori D. Williams
Purifying DNA from gel slices Christoph H. Winter
Autoclaving Reagents David Winterbourne
Cell Lysing Detergent Stefan Wirtz
Isolating RNA without using DNase?? Stefan Wirtz
Purifying DNA from gel slices Stefan Wirtz
Inclusion of restriction sites in PCR primers Harry Witchel
Expression using metallothonein promoter in CHO cells Harry Witchel
multimers in plasmids Robert Woodward
Amphotericin B in plant tissue culture? Steve Wylie
DD-PCR Youhua Xie
Nuclear extracts/suspensions Youhua Xie
Wanted : protocol for bandshifts Youhua Xie
Reverse Northern? Youhua Xie
DNA size markers Youhua Xie
Concentration of DNA Youhua Xie
help dd-PCR Youhua Xie
rTth reverse transcriptase kit Z. YU
Purifying DNA from gel slices Mayumi Yagi
GST-fusion protein Xianlong Yang
supF selection, pcDNAI-neo Ian A. York
pBluescript mcs addition Taek H. You
How to wash glassware for using with mRNA experiment Taek H. You
Nonisotopic gel shift assay David Tak Yan Yu
Protocol or tips on physical map of gene Yan Yu
help in physical map of DNA Yan Yu
DNA strands, WHO'S WHO. Zhongtang Yu
Ti Plasmid Bill Zabaronick
Anti-proteasome-antibodies? Matthias Zeiner
Eukaryotic expression system Fang Zhao
electron microscopy - need help Fang Zhao
Storm vs Molecular Imager Marieke R. Koedood Zhao
in situ hybridization Marieke R. Koedood Zhao
Help Zhiyong Zhao
I need your help Liu Zhen
I need your help Liu Zhen
functional expression of Zif268 in E.coli Zhao Zhihu
source of wheat germ please Christian Zwieb
source of wheat germ please Christian Zwieb
in situ hybridization ae at dna.bio.warwick.ac.uk
good Reverse Transcriptase... man called aerodynamics...
Cell cultures. Requesting information ajcm.acia at bcn.servicom.es
good Reverse Transcriptase... eric anderson
Electroporaton cuvettes - can you recycle them? eric anderson
KlentaqLA extra A eric anderson
checking ligations by PCR, help. eric anderson
Green fluorescent protein vector eric anderson
In situ PCR of bacterial colonies? eric anderson
Help to find Methicillin eric anderson
make a hot probe by PCR eric anderson
For sale Digital Autoradiograph ando
Bovine Serum Albumin in PCR angelpet4u at aol.com
Bovine Serum Albumin in PCR angelpet4u at aol.com
Bovine Serum Albumin in PCR angelpet4u at aol.com
Bovine Serum Albumin in PCR angelpet4u at aol.com
Bovine Serum Albumin in PCR angelpet4u at aol.com
Bovine Serum Albumin in PCR angelpet4u at aol.com
Bovine Serum Albumin in PCR angelpet4u at aol.com
Hybridization of colony lifts annie.bourdeau at utoronto.ca
BL21(DE3) pLYSe/s competent cells ashaw5 at aol.com
oligonucleotide probe for bikunin dr. bev
computer program for searching consensus samir bhagwat
Long RT-PCR bionbkx at panther.gsu.edu
GST-fusion protein biotec54 at aol.com
difficulties obtaining genomic DNA bogdanov at post.uv.es
difficulties obtaining genomic DNA bogdanov at post.uv.es
Prep of DNA for electroporation brett
acetone precipitation of samples (for SDS PAGEs) brett
Electroporation cuvettes brett
fluorescent RNA stain for microscopy brett
TE Buffer for RNA brett
Cre RECOMBINATION jackie brittingham
differential display RT-PCR bruce
help dd-PCR bruce
how dangerous is ethidium bromide Stefano casalotti
RNA storage chriso at dfrc.wisc.edu
densitometry hardware chriso at dfrc.wisc.edu
Differential Display Kit - Display Systems cisca at KAN.RPA.CS.NSW.GOV.AU
Best way to clean cuvettes? clkstanley at postoffice.worldnet.att.net
transfection of HL60 cmy
E.coli strain davesmith at bioch.tamu.edu
MPSS delagoutte
Purifying DNA from gel slices orlando dominguez
her-2/neu donnap at utmdacc.uth.tmc.edu
chloramphenicol-sepharase harold j drabkin
RNA extraction help ewmccar at aol.com
Concentration of DNA fang at SASK.USASK.CA
T/A cloning of PCR products gotow lisa fujiko
T/A PCR cloning gbga14 at udcf.gla.ac.uk
request Microfluidizer and Zeocin gerard at adnc.com
Downloading PC-Rare Files Rémy griffais
Uracile in degenerated primer guiguen
specificity of taq DNA pol? hbkg at hitel.kol.co.kr
specificity of taq DNA pol? hbkg at hitel.kol.co.kr
specificity of taq DNA pol? hbkg at hitel.kol.co.kr
specificity of taq DNA pol? hbkg at hitel.kol.co.kr
specificity of taq DNA pol? hbkg at hitel.kol.co.kr
hepatitis B virus? hbkg at hitel.kol.co.kr
transcription? hbkg at hitel.kol.co.kr
Sequence analysis??? hu at ALLEGHENY.EDU
Soubilizing protein pellet for liquid scintillation counting huang at sun5.ibp.ac.cn
Doxycycline stable in H2O? iddavis at vms.cis.pitt.edu
Staining agarose gels Administrador de iihema.fmed.uba.ar
Staining agarose gels Administrador de iihema.fmed.uba.ar
Insoluble GST-fusion ijiwaru at wheel.dcn.davis.ca.us
Phagemid-Kit for Kunkel Mutagenesis commercially available ? j.langner
E.coli BW313 j.langner
E.coli BW313 j.langner
rna removal from plasmid preps with Li Cl precipitation daniel jacobs
2.4 kb PCR daniel jacobs
polysome associated RNA isolation from mouse tissues jain at merle.acns.nwu.edu
Linkage Mapping Software needed jamiem at puccini.crl.umn.edu
Bovine Serum Albumin in PCR jenova at aol.com
yeast two hybrid library jhughes
Anyone know the best OD value for protein induction in S. cerevisiae? jlight at SICKKIDS.ON.CA
Looking for an program to predict antigenic regions in a protein sequence jlight at SICKKIDS.ON.CA
help on transient peptide features joaroyo at samba.cnb.uam.es
transfection of HL60 jovermeyer at shrsys.hslc.org
HeLa or Liver for GST-fishing? jstrassw at OPAL.TUFTS.EDU
attachment HEK-293 cells jstrassw at OPAL.TUFTS.EDU
Green fluorescent protein vector jstrassw at OPAL.TUFTS.EDU
Wanted:Method for transfection of primary human fibroblasts jstrassw at OPAL.TUFTS.EDU
Storm vs Molecular Imager jstrassw at OPAL.TUFTS.EDU
Q: pGL3basic: Ligation TIME and TEMPERATURE?? jstrassw at OPAL.TUFTS.EDU
Green fluorescent protein vector jstrassw at OPAL.TUFTS.EDU
Method: Electroporate COS-7 jstrassw at OPAL.TUFTS.EDU
Q: pGL3basic: Ligation TIME and TEMPERATURE?? jstrassw at OPAL.TUFTS.EDU
Method: Electroporate COS-7 jstrassw at OPAL.TUFTS.EDU
Library insert DNA jwobrien
Why freeze autorads? klenchin at facstaff.wisc.edu
Why freeze autorads? klenchin at facstaff.wisc.edu
Method: Electroporate COS-7 klenchin at facstaff.wisc.edu
Help on h-ras codon 61 mutation needed! Does anyone know of a cell line that has this mutation? kretscsm at ucbeh.san.uc.edu
AA sequence randomization? campanelli lab
Differential hybridisation charles lawrie
Quik change insertions lidaniel
Plasmid recovery from yeast lobreaux at ensam.inra.fr
ds oligo purification lobreaux at ensam.inra.fr
Drosophila Schneider cells expression system.... mac-biocomputing1user
Drosophila Schneider Cell Expression System... mac-biocomputing2user
Purifying DNA from gel slices mac-biocomputing2user
WHY DON'T WE FREEZE 35-S AUTORADS ? mac-biocomputing2user
WHY DON'T WE FREEZE 35-S AUTORADS ? mac-biocomputing2user
microsatellite amplification problem maria
subcloning of large DNA fragments (1,7 Kb) marieeve.paquet at utoronto.ca
ThermoSequenase Manual Sequencing Kit from Amersham jose manaligod md
Library insert DNA jose manaligod md
Non-isotopic gel shift assay heidi moss
ABI 310 mullenlynne at .jsei.ucla.edu
Storm vs Molecular Imager mullenlynne at .jsei.ucla.edu
(none) swati pandya
ELISA and fibronectin pena at post.uv.es
S: Labeled ribonucleotide prbuzz at aol.com
Indirect Tyramide amplifications probes at cytocell.co.uk
Codon preferences probes at cytocell.co.uk
Electrophoresis of cholesterol radavis at VM.SC.EDU
BAC vectors/libraries rbrosch
pcr ricete4 at wfu.edu
Two PCR questions rk6n at VIRGINIA.EDU
Labelling with Terminal transferase rk6n at VIRGINIA.EDU
Help with Lysis Buffer 4 adherent cells please ! runting at wehi.edu.au
Antibody to E.coli peripheral membrane protein rvisvanathan
in situ hybridisation s.pritchard
betaine/sorbitol questions p.j. van santbrink
T/A PCR cloning severson at uoneuro.uoregon.edu
differential display RT-PCR sunil shaw
antisense technology? slavemaster
DNA Quantification slavemaster
elution of oligo probes from PA gel snk-mrk
capsicum stability test needed spikef at aol.com
long pcr susan stepp
Plasmid recovery from yeast vlad svetlov
How to use freeze-dried E.coli shimokawa teruhiko
storm Matt thomas
QT6 cells??? timmer at physio.emory.edu
Primer deletion usrer
TEV-protease cDNA/RNA search..... vanderzandt at embl-heidelberg.de
TUNEL labeling of fixed tissue: Carnoy's vs paraformaldehyde vilimf01 at mcrcr6.med.nyu.edu
Northern blot trouble vilimf01 at mcrcr6.med.nyu.edu
purifying dna from gel slices vilimf01 at mcrcr6.med.nyu.edu
purifying dna from gel slices vilimf01 at mcrcr6.med.nyu.edu
Effect of TE on ligations? vilimf01 at mcrcr6.med.nyu.edu
Problem with RACE vilimf01 at mcrcr6.med.nyu.edu
DTT wudc at NIC.BMI.AC.CN
DD-PCR wudc at NIC.BMI.AC.CN
Neutrophil isolation wwu
Rna gel wwu
maxam-gilbert sequencing cindy yamamoto
pGL3 is terrible!!! z
Fusion protein activity slavemaster z
Cell Lysing Detergent slavemaster z
Knock-Out, which vector slavemaster z
glass tubes for hyb.-oven needed slavemaster z
rna removal from plasmid preps with Li Cl precipitation slavemaster z
checking ligations by PCR, help. slavemaster z
Riboprobes, Northerns, and high background slavemaster z
WHY DON'T WE FREEZE 35-S AUTORADS ? slavemaster z
checking ligations by PCR, help. slavemaster z
Knock-Out, which vector slavemaster z
WHY DON'T WE FREEZE 35-S AUTORADS ? slavemaster z
hybridization buffer slavemaster z
Exo-III kit, which company ? slavemaster z
WANTED: Marking Pen for Autorads ying zhang
storm zinc
Storm vs Molecular Imager zinc
How to show protein interaction? zinc

Last message date: Sat Nov 30 13:56:23 EST 1996
Archived on: Sun Mar 6 21:14:22 EST 2005

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