DNA in Inclusion Body Preps

Thomas Cameron cameron at risotto.mit.edu
Tue Oct 1 19:51:36 EST 1996


We are refolding proteins from E. coli inclusion bodies.
Often in our preps we get large amounts of residual chromosomal DNA
despite large amounts of DNase added during lysis.

Does anyone know how to get rid of chromosomal DNA from Urea-solubilized
inclusion bodies?

Thanks
Lihui Xu

P.S. we've tried DEAE, hydroxyapatite, polyamine matrices already.



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