In article <52rve5$b4e at gazette.bcm.tmc.edu>, ah690549 at bcm.tmc.edu (Annette
C. Hollmann) wrote:
> Does anyone know of a supplier that still sells Genetron ? It is a freon
> reagent used to purify viral particles, and due to environmental
> regulations it is becoming more and more difficult to obtain.
>> Alternatively, does anyone know of a more environment-friendly
> alternative to Genetron? We need to purify SV40.
>> Thanks :-)
>ah690549 at mbcr.bcm.tmc.edu
I don't know if it is any friendlier to the environment but you might wish
to try 1,1,2-trichloro-1,2,2-trifluoroethane (Fisher has it for sure) - we
used it years ago while purifying adenovirus particles.
Karl the hepB guy
tyr-2 at bones.biochem.ualberta.ca