Genetron (freon)

[Karl Fischer]

In article <52rve5$b4e at gazette.bcm.tmc.edu>, ah690549 at bcm.tmc.edu (Annette
C. Hollmann) wrote:

> Does anyone know of a supplier that still sells Genetron ? It is a freon 
> reagent used to purify viral particles, and due to environmental 
> regulations it is becoming more and more difficult to obtain.
> 
> Alternatively, does anyone know of a more environment-friendly 
> alternative to Genetron? We need to purify SV40.
> 
> Thanks :-)
> 
> Annette
> ah690549 at mbcr.bcm.tmc.edu

I don't know if it is any friendlier to the environment but you might wish
to try 1,1,2-trichloro-1,2,2-trifluoroethane (Fisher has it for sure) - we
used it years ago while purifying adenovirus particles.

Cheers

Karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca