How much ampicillin?

[dreamer at super.zippo.com]

"Don Walthers" <walt9693 at uidaho.edu> wrote:

>the antibiotic timentin (sp?) can be used as an amp replacement to take
>care
>of satallites found with long incubations. i don't know of a commercial
>source

>Tori D. Williams <twilliam at BIOMED.MED.YALE.EDU> wrote in article
><Pine.PMDF.3.91.960930152428.115586C-100000 at BIOMED.MED.YALE.EDU>...
>> I use 100 ug/ml and get stallites. Someone suggested 150 ug/ml. I tried 
>> it and I still got colonies don't remember if I got satallites or not but

>> use 100 out of habit.
>> 
>> I think I only get satallites when the plates are in the incubator longer

>> than 16 hours.
>> 

We use carbenicillin 200 ug/ml for pBR and pUC plasmids and don't get
any satellites even after 24h of incubation. You can buy it from
Sigma.