automated dna sequencing

Rick Thorne birft at cc.newcastle.edu.au
Thu Oct 3 00:44:10 EST 1996


Aarthi,

Two buffer chambers are used for the purposes of electrophoresis.  The 
upper chamber acting as the anode and the lower chamber as the cathode.  
The slab gel itself acts as a gate and draws current between the two 
chambers promoting the electrophoresis of the DNA fragments.  The 
average pH of the TBE buffer used in the system is around 7.5 and 
formamide promotes the denaturation of DNA molecules from double 
stranded to single stranded.

Regards,

Dale





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