radiolabelled supercoiled plasmid

Eric Lader lader at
Wed Oct 2 21:07:52 EST 1996

In article <3251A3FA.998 at> Gail Otulakowski,
gotulak at writes:
>I wish to label plasmid with 32P, but I can't use nick translation or 
>random priming as these will affect the conformation and I need to look 
>at supercoiled plasmid. 

maybe label it non isotopically with photo or Psoryln biotin?

          **my opinions are just that**
        Eric Lader Ph.D.	Senior R&D Scientist,  
                          Ambion Inc.
Unique tools for Molecular Biology Research

Phone (800) 888-8804       Fax (512) 445-7139
				  (512) 445-6979       lader at

More information about the Methods mailing list