stable transfection of HepG2 cells

[Gerhard Püschel]

Can someone help me?

One aim of my studies is stable transfection of HepG2 cells with a 
plasmid containing a receptor cDNA. I did this one time with success using 
Ca-phosphate method but this method did not work with two other plasmids. Is 
there a better method for stable transfection of HepG2 cells, which 
concentration of G-418 is the best for selection and what is the best way to 
handle the cells during selection. 

If someone has experience with stable transfection of HepG2 cells 

please answer me. 

Your Frank Neuschaefer-Rube
Göttingen, Germany