I wish to label plasmid with 32P, but I can't use nick translation or
random priming as these will affect the conformation and I need to look
at supercoiled plasmid. I think I will have to add radiolabelled
inorganic phosphate to bacteria grown in minimal media - so two questions
- has anyone grown E coli in M9 minimal for plasmid preps and will it
make my yield lousy? Also, M9 is normally buffered with phosphate - I
can buffer with Tris instead but what are E.coli's minimal requirements
for phosphate so I know how much hot stuff to add?
