Differential display

Olivier Gandrillon ogandril at cri.ens-lyon.fr
Thu Oct 3 03:24:43 EST 1996


Hi

I would suggest subclone in a plasmid vector and then test the inserts 
by a reverse Northern technique (see for example Liu C and Raghotama, KG 
(1996) BioTechniques 20, 576-580.). I have no personnal experience of 
this but this is how I would proceeed.

Hope this helps

Olivier

Akihiro Yamaguchi wrote:
> 
> I have isolated bands from differential display gels.  I understand that each
> band might contain more than one PCR product.  I would appreciate advice on
> what is the best way for screening.
> 
> Best wishes,
> David Yu
> UCLA
> dtyyu at ucla.edu



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