automated dna sequencing

[APadmanabh]

Hi,

I'd like someone to answer the following questions:
1. How to TEMED, acrylamide and APS polymerize to form the gel?
2. EDTA is a chelating agent.  What is a chelating agent?
3. Why is dna denatured before it is loaded?
4. Why is the dye terminator method considered more robust?
5. When you call bases, during analysis, what exactly are you doing?

Regards,

Arti