Hi, In the so far published papers on the one hybrid people are always using several repeats of the tested DNA binding site before a minimal promoter. Is this not unstable regarding the recombination efficiency in yeast? All usefull critical comments are welcome! Sincerely, Geert Dr. Geert Berx K.L. Ledeganckstraat 35 9000 Ghent Belgium Geert at lmb1.rug.ac.be