Making 2% skimmed milk in PBS
Richard P. Grant
rpgrant at molbiol.ox.ac.uk
Thu Oct 3 16:59:50 EST 1996
In article <Robert_Willows-0310961046370001 at 18.104.22.168>, Robert_Willows at brown.edu (Robert Willows) writes:
> We routinely use 15mM Sodium Azide in our 2% skim milk to stop bacterial
> growth. Although, I don't think you can do this if you use a HRP
> conjugated secondary because I think the azide is inhibitory.
If you wash your filters properly, it's OK.
Azide is not necesssary for a 60' block.
Richard P. Grant MA DPhil rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
No wonder I can't go to parties anymore.
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