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Making 2% skimmed milk in PBS

Richard P. Grant rpgrant at molbiol.ox.ac.uk
Thu Oct 3 16:59:50 EST 1996

In article <Robert_Willows-0310961046370001 at>, Robert_Willows at brown.edu (Robert Willows) writes:
> We routinely use 15mM Sodium Azide in our 2% skim milk to stop bacterial
> growth. Although, I don't think you can do this if you use a HRP
> conjugated secondary because I think the azide is inhibitory.

If you wash your filters properly, it's OK.
Azide is not necesssary for a 60' block.

Richard P. Grant MA DPhil      rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
No wonder I can't go to parties anymore.

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