In article <Robert_Willows-0310961046370001 at 128.148.57.20>, Robert_Willows at brown.edu (Robert Willows) writes:
> We routinely use 15mM Sodium Azide in our 2% skim milk to stop bacterial
> growth. Although, I don't think you can do this if you use a HRP
> conjugated secondary because I think the azide is inhibitory.
If you wash your filters properly, it's OK.
Azide is not necesssary for a 60' block.
R
--
Richard P. Grant MA DPhil rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
http://users.ox.ac.uk/~lady0266
No wonder I can't go to parties anymore.
