Making 2% skimmed milk in PBS
Richard P. Grant
rpgrant at molbiol.ox.ac.uk
Thu Oct 3 16:59:50 EST 1996
In article <Robert_Willows-0310961046370001 at 126.96.36.199>, Robert_Willows at brown.edu (Robert Willows) writes:
> We routinely use 15mM Sodium Azide in our 2% skim milk to stop bacterial
> growth. Although, I don't think you can do this if you use a HRP
> conjugated secondary because I think the azide is inhibitory.
If you wash your filters properly, it's OK.
Azide is not necesssary for a 60' block.
Richard P. Grant MA DPhil rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
No wonder I can't go to parties anymore.
More information about the Methods
Send comments to us at biosci-help [At] net.bio.net