How much ampicillin?

Joe Boutell boutell at cf.ac.uk
Thu Oct 3 09:24:00 EST 1996


dreamer at super.zippo.com wrote:
>"Don Walthers" <walt9693 at uidaho.edu> wrote:
>
>>the antibiotic timentin (sp?) can be used as an amp replacement to take
>>care
>>of satallites found with long incubations. i don't know of a commercial
>>source
>
>>Tori D. Williams <twilliam at BIOMED.MED.YALE.EDU> wrote in article
>><Pine.PMDF.3.91.960930152428.115586C-100000 at BIOMED.MED.YALE.EDU>...
>>> I use 100 ug/ml and get stallites. Someone suggested 150 ug/ml. I tried 
>>> it and I still got colonies don't remember if I got satallites or not but
>
>>> use 100 out of habit.
>>> 
>>> I think I only get satallites when the plates are in the incubator longer
>
>>> than 16 hours.
>>> 
>
>We use carbenicillin 200 ug/ml for pBR and pUC plasmids and don't get
>any satellites even after 24h of incubation. You can buy it from
>Sigma.
>
>
Yep, we use carbenicillin, but only at 50 ug/ml. However, it is more 
expensive than ampicillin.




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