Best oligo purification?

Mary Ann Cushman cushmam at okstate.edu
Thu Oct 3 18:14:49 EST 1996


Hi-
6% MetaPhor (FMC) agarose (1X TBE, 0.5% EtBr) with a large preparative 
comb, followed by electroelution, desalting and concentration with 
centricon. Easier than acrylamide sequencing gel followed by crush and 
soak. MAYBE a little faster.
P.S. Add the MetaPhor to RT 1X TBE, stir in, then heat slowly to boiling 
and clearing, since it boils over too easily in microwave.
I have used this successfully for 20-50-mers.
Good luck!
Mer 





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